Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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Transformation of Bacterial Cells with Plasmid DNA Introduction: Transformation refers to the process in which the cell integrates foreign DNA to its genetic code‚ meaning it takes the genes and incorporates them into the cell’s current DNA. Cells that can do this naturally‚ most commonly bacteria and archea‚ are known as competent. The bacteria E. coli do not have high transformation competence under normal conditions‚ but can be manipulated to produce better results using
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Capitol 900 Court Street Salem‚ Oregon 97301-4047 Dear Governor John Kitzhaber: I am writing to you regarding state facts and restrictions on abortions in Oregon. A majority of states require parental involvement in a minor’s decision to have an abortion. I agree with most laws in Oregon concerning abortion rights; however‚ my concern is the law regarding the restriction or lack of‚ when it comes to parental involvement required for abortion‚ there is none. Most of these states only require the consent
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Identification of Mystery Plasmids Abstract Plasmids serve as important tools in genetics and biotechnology labs‚ where they are commonly used make many copies of a particular gene. The aim of this experiment was to identify three mystery plasmids based upon their characteristics; such as size‚ antibiotic resistance‚ lacZ profile and conjugative properties. The results obtained showed that plasmid number2 was the pDSK519 plasmid and its size was 26229.58 Bp. Plasmid pDSK519 also was found to be
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The Trifle Isolation Have you ever felt as though you were alone? Susan Glaspell wrote a play called Trifles. The story tells of the untimely death of Mr. Wright and the subsequent investigation. The investigation is being overseen by the county attorney‚ the sheriff and Mr. Hale who have brought their wives along to retrieve Mrs. Wright’s personal effects from the home. Everyone suspects Mrs. Wright of the death. The story has many themes but the one that stands out to me is isolation. This unfortunate
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BASE ISOLATION: FRICTION PENDULUM SYSTEM SARNOT ANUP A.‚ JAIN KRUTIK P‚ CHOUDHARY JUNAID A‚ U.G.Students Department of Civil Engineering D.M.College of Engineering Sector-3‚ Airoli‚ New Mumbai- 400 708 Abstract: The Base Isolation is one of the passive control techniques to reduce the earthquake effect on the structure up to negligible level. Base isolation system are‚ Elastomeric Bearing‚ Sliding Isolation System and Combined Systems. Elastomeric bearing has a problem of tearing of rubber
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Isabella Correa Pr. ENC 04/16/2013 Research Paper: Gun Control Restrictions According to the 2010 statistics presented by the United Nations Office on Drugs and Crime‚ (UNODC‚) in Great Britain‚ Sweden‚ Switzerland‚ Australia‚ Canada‚ and Japan‚ a total of 294 people were murdered by firearms. In the United States more than 22‚000 people were murdered by firearms by the end of the year. The difference that exists among these countries and The United States is that in these countries
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Halloween is a traditional thing for every family‚but should we ban it for the older people and only have a tradition for the young kids? If we put an age on Halloween not many people will be out. What else would our society put an age restriction on? Teens really can’t do anything now and days ‚because our society puts a ago on everything.We shouldn’t put a age on trick or treating because No one would really be out around 8 and Halloween is a traditions . Halloween is a night where everyone comes
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reddish brown‚ dry corky rot. Heavy infection can give rise to total loss of the tubers. Light infections can occur and are difficult to detect. Media and antibiotics for isolation of Phytophthora from diseased plant tissue and soil The Oomycetes are not true fungi‚ and therefore special techniques are required for their isolation. Most species of Phytophthora grow rather slowly in vitro compared with saprophytic fungi and bacteria. In addition‚ bacterial populations need to be kept low because they
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Restrictions Upon Women (Final Draft) In the article “Size 6: The Western Women’s Harem”‚ Fatema Mernissi talks about how Western beauty standards harm and embarrass the female population even so as the veil does the same in extremist nations‚ if forced by authorities. She explains how the Eastern countries do not have such a rigid standard of beauty and how men are simply not part of fashion‚ in contrary to the West where fashion is used by men to control what women wear. She does this by relating
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