Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
Premium Bacteria Molecular biology Protein
Freedom or Restriction Imagine living in the 1860’s as an African-American. You are beginning to get your rights to vote and your gaining your freedom. In1860‚ the census revealed that the national population was 31‚000‚000. Out of the 31 million‚ only 4.5 million of them were black (14percent) Only 221‚000 free blacks lived in the north which is 20 percent of the black population. By the end of the 1800’s‚ most Northern Blacks were free. But did Northern blacks really free or did they have more
Premium Black people African American Race
Over Immigration Restriction The term immigration refers to moving from your native country and coming to a foreign land for the purpose of a permanent residence and searching for greener pastures. There are several arguments by scholars about immigration restrictions to the United States. They had several similarities supporting immigration restrictions. Prescott Hall‚ Robert Ward‚ Frank Wright‚ Frank Fetter and John Mitchell all argued supporting the immigration restrictions. Immigrants
Premium Immigration to the United States United States Immigration
used to introduce the gene‚ usually a plasmid or bacteriophage‚ is called the vector. Ideal vectors have high rates of replication. Gene cloning is an important procedure in genetic engineering that allows us to produce a large amount of a useful gene product: for instance‚ insulin can be made for diabetic individuals by cloning genes from pancreatic cells.(Wikipedia 15/11/2014) Cloning vector: A cloning vector is a small piece of DNA‚ taken from a virus‚ a plasmid‚ or the cell of a higher organism‚
Premium Molecular biology DNA Plasmid
Isolation can be okay if the person realizes that being social and getting work done is still important. For many however‚ watching 6 hours of Grey’s in the comfort of a bed can easily outweigh going out to coffee to do homework with peers. Isolation is defined by a list of attributes that describe what a person who is isolated may look like. These attributes include having
Premium Antisocial personality disorder Psychopathy Conduct disorder
I believe that World Wide Web restrictions should not be allowed. I believe that they are not helpful to the people that use the World Wide Web. I feel that the restrictions on the World Wide Web at school are too strict. At school most sites you try to view are prohibited and they are totally harmless sites. I feel that at school the only restrictions that should be put on the World Wide Web are restrictions to pornographic sites. Even these sites should not be blocked because some harmless
Premium World Wide Web
Should the United States put more restrictions on gun ownership and use? Gun control is not one issue‚ but many. It is a very delicate issue with many different sides and viewpoints. Gun control is an ongoing argument that will never be settled. Some people believe gun control is a crime issue and others believe it is a right’s issue. Within each of these issues are those who want more gun control and those who want less. Gun control will not be a benefit to society‚ but rather a downfall. The United
Premium Firearm Gun politics in the United States Gun
individual property‚ or paid a stated amount in taxes. A huge problem with that is this only allowed around seventy of white males to vote. But because of this reason‚ cities only had a percentage of forty to fifty percent. However‚ there were other restrictions on voting‚ which include religion. For example‚ Catholics and Jews were both banned to vote in some colonies. Right after the American Revolution‚ some states‚ replaced property qualifications with taxpaying requirements. This obviously caused
Premium American Revolution United States United States Declaration of Independence
ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
Premium Bacteria Microbiology
Driving Restrictions There are many accidents everyday that are costing people their lives. My young brothers and most definitely my grandparents would probably kill me for saying so‚ but there should be some changes made to the laws concerning the age requirements of drivers. If the State of Ohio changed the age requirement of operating a vehicle to between 18-70 years of age‚ our roads would be much safer to drive on. If you had told me when I turned sixteen that I couldn’t get my license
Premium Driver's license Old age Driving