Assay of succinate dehydrogenase of after isolation of mitochondria in Cauliflower (Brassica oleracea) using differential centrifugation. Kelly M. Messick‚ Rebecca Conner Department of Biological Sciences‚ Salisbury University‚ Salisbury‚ MD‚ 21801 U.S.A Address for correspondence: Kelly M Messick Department of Biological Sciences Salisbury University Salisbury‚ MD 21801 Phone: 410-546-2060 Fax: 410-543-6433 e-mail: km96536@gulls.salisbury.edu Running title: Assay of succinate dehydrogenase
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Electron Transport Chain The first step in the electron transport chain process is for the NADH2 produced during glycolysis‚ the intermediate step‚ and the citric acid cycle to be attracted to Complex I (FMN ·FeS)due to its high affinity for NADH2. This attraction pulls NADH2 to Complex I (NAD dehydrogenase) and the two electrons from H2 are pulled off by the FeS (ferrous sulfate) leaving two H+ ions and NAD+. These molecules repel each other and this results in the NAD+ being recycled
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March 5‚ 2013 Biology 1111 Term Paper Glycolysis and the Citric Acid Cycle Glycolysis occurs in the cytoplasm outside the mitochondria‚ it is a metabolic pathway that is the breakdown of glucose (C6) and ends with 2 pyruvate (C3) molecules. Energy is invested to activate the glucose‚ 2 ATP are gained‚ and oxidation results in NADH‚ which will be used later for additional ATP production. Glycolysis is divided into (1) the energy-investment step‚ when ATP is used; and (2) the energy-harvesting
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11 Physical Education Unit 2‚ 2012 - Touch & the Energy Systems Inocentes‚ Steven 11 Physical Education Unit 2‚ 2012 - Touch & the Energy Systems Inocentes‚ Steven Table of Contents 1.0 Introduction____________________________________________________2 2.0 Energy Systems used in touch______________________________________2 3.1 Fatigue and Performance during Touch________________________3 3.0 Recommendations – Tactics / Strategies_____________________________3
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THE ROLE OF PROTEIN MISFOLDING AND AGGREGATION IN BSE When a protein misfolds it changes its behavior and function. If it becomes hydrophobic after once being polar. The properties and functionality of the protein are no longer useful to the organism and disaster results. PrPSc is hydrophobic‚ it avoids water inside of the cell…it attracts and attaches other proteins to misfolds and become hydrophobic …Misfolding spreads because the PrPSc act as chaperone proteins to convert PrPc TO PrPSc and
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CHAPTER I INTRODUCTION A lot of people have been getting into the weight-loss bandwagon. Nowadays there are numerous supplements and drinks that promise significant weight loss after a certain amount of time following regular consumption. One famous chemical currently advertised is L-carnitine. The substance is water soluble which oxidizes and transports long chain fatty acids into the mitochondrion (Dokmeci et al.‚ 2005; Gulcin‚ 2006)‚ transeterifies and acetyl-CoA‚ oxidizes branched chain
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Muscle contractions happen when muscle fibers are stimulated‚ which can cause one of many types of contractions. Isometric contractions‚ which means that tension happens in the muscle but there is no change in muscle length‚ therefore there is no movement of the muscle itself. An example of Isometric contractions would be strength training‚ such as holding a weight still‚ which happens in the biceps brachii. The biceps brachii the gets more tension‚ but the muscle length stays the same. As for isotonic
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The aim of this experiment is to better understand the process of fermentation of yeast in different concentrations of sucrose. The experiment worked with yeast and sugar (sucrose and glucose) to determine the rate of fermentation by testing the pressure of C02 in the test tube. The experiment tested the metabolic capability of yeast anaerobically meaning no oxygen was present (this was ensured by the thin layer of oil on the top of the solution). This means that the metabolic rate of the yeast could
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HES 403 Exam 2 Study Questions Draw a graph that illustrates whole-body oxygen consumption as an individual progresses from rest‚ to 45 min bout of exercise at 60% VO2max‚ and through a complete recovery phase. Explain what is occurring during each of these stages in relation to ATP and oxygen supply and demand. Explain 4 ways that metabolic substrates (“fuels”) are made available to working muscles during prolonged aerobic exercise. What hormones contribute to these effects? What is
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Link reaction The link reaction takes place in the mitochondrial matrix and its main function is to turn the pyruvate into acetate for the kerb cycle. During the link reaction the pyruvate molecule undergoes decarboxylation and dehydrogenation‚ the enzymes pyruvate decarboxylase and pyruvate dehydrogenase remove the carboxyl group (which becomes a carbon dioxide molecule) and removes the hydrogen atoms from the pyruvate molecule. The coenzyme NAD accepts the two hydrogen atoms and becomes reduced
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