MICROBIOLOGY COURSEWORK The effect of antibiotic concentration on bacterial growth Introduction Null Hypothesis- Increasing the antibiotic concentration has no effect on bacterial growth. Experimental Hypothesis- Increasing the antibiotic concentration decreases bacterial growth. Scientific Theory Bacteria are prokaryotes and can be identified by their shape. Spherical bacteria are called cocci‚ rod shaped bacteria are called bacilli‚ spiral shaped bacteria are called spirilla and
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FeSCN2+ Kc = [FeSCN2+]/{[Fe3+][SCN-]} 3) Objectives: To determine the equilibrium constant; Kc of the reaction between Fe3+ and SCN- ions 4) Materials: Computer‚ Vernier computer interface‚ Logger Pro‚ Vernier colorimeter‚ 1plastic cuvette‚5 test tubes of 20x150mm‚ thermometer‚ 0.0020M KSCN‚ 0.002M Fe(NO3)3( IN 1.0M HNO3)‚ 0.2M Fe (NO3)3 (in 1.0M HNO3)‚ 4 pipets‚ pipet bulb‚ tissues. 5) Safety measure: Wear goggle and gloves to protect against
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A candle (C25H52) is a solid block of wax with an embedded wick‚ which is lit to provide light‚ and sometimes heat‚ and historically as a method of keeping time. Candles have appeared as a source of fine and ultrafine particles in indoor air (e.g. Hussein et al. 2006). Fine et al. (1999)‚ found high Organic Carbon (OC) emissions connected to the extinction of the candle and high EC concentrations when the flame was burning unsteady. In the mid 1800s there were two major developments in the
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find the optimum concentration for maximum absorbance. C = A/ ε Methods: The two reagents used are Bromophenol blue and Methyly orange. Experiment 1: 1 Set up the colorimeter at 400nm‚ measure the absorbance of both reagents against distilled water‚ this will be used as a blank (make sure to zero the colorimeter after each interval) 2 Repeat this procedure at 20nm from 420nm to 700nm 3 After getting a range of results make further readings in smaller intervals to precisely locate
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What is the Effect of the Colour of Light on the Rate of Photosynthesis? By Amelia Rowan‚ 12SDa Aim To discover whether the colour of the light source has any effect on the rate of photosynthesis of a sample of algae. Hypothesis It was predicted that the colour of the light source would have a very slight effect on the rate of photosynthesis. It was further predicted that when the sample was placed under the green light‚ the rate of photosynthesis would be the lowest. This hypothesis was arrived
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LED MARKET OVERVIEW AND FORECAST ‐ INDIA Electric Lamp & Component Manufacturers’ Association of India Taipei – March 12‐15‚ 2012 Lighting Industry in India • Performance year 2011 Industry growth Growth per annum 10000 17% LIGHTING INDUSTRY IN INDIA ‐ GROWTH TREND CHART LIGHTING INDUSTRY IN INDIA GROWTH TREND CHART 12% 9000 9% 15% 8000 12.5 % 7000 6000 13% 9290 7994 7167 US $ 2 Billion 6567 5711 5060 5000 4500 Rs. Crores
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to it‚ the solution will turn blue-black. Aim: The aim for this experiment is to investigate how the different buffer solutions work on the enzyme amylase. This will be investigated by using iodine and by timing the experiment using the colorimeter and the program Loggerlite. Hypothesis: The pH denatures enzymes‚ and since amylase is an enzyme‚ my hypothesis is that the pH will change the shape of the active site of the enzyme‚ so the substrate will not be able to fit in anymore.
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An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
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boiling tube racks Thermometer (one for each water baths) Colorimeter Cuvettes Stopcocks Distilled Water Pipettes Small measuring cylinder Independent variable: Temperature (°C) – this will be controlled by the water baths and each water bath will have a thermometer placed inside. The temperature will range from 0°C to 70°C and will go up in 10. Dependent variable: % of Absorbance- the absorbance will be measured by the colorimeter which will have 2cm3 of the dye solution to make it a
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Practical Assessment Planning AIM Beetroot cells contain a red pigment‚ which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. The outer of the cell is also surrounded by a membrane‚ which again helps contain the pigment inside the cell. In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of the beetroot
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