Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the experiment
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digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web. 10 Dec. 2012. Research Question: Does changing the pH of pepsin affect the ability to break down the protein (mass of jello). Variables: independent- Ph level dependent- The mass of the jello
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The relatively close pH levels of Tap Water‚ Spring Water‚ Flavored Water‚ and Seltzer Water. Introduction This experiment was performed to investigate the following hypothesis: The following four different types of drinking water (spring water‚ seltzer water‚ tap water‚ and flavored water) we test will relatively have the same pH level. Our group decided to test different types of drinking water to see if a pH level of tap water is comparable to other types of water. Many people believe
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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investigate how pH affects the rate of protein digestion Hypothesis: I predict that since pepsin breaks down the material the quickest at a pH of 2 in the stomach‚ test tubes #3 will reduce the greatest because it has pepsin to further break down the egg. Whereas the other test tubes will have a smaller reduction. I think test tube #2 will have the second largest reduction rate and then #6 because they all will have a greater rate at breaking down the material‚ Then‚ I predict the test tubes that did
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EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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pH and Living Systems I. Purpose: To observe the effects of pH change on an organic molecule. II. Materials: pH paper Droppers Ammonia Beakers (50ml) Paper towels water Glass stirring rods lemon juice forceps III. Procedure: Part 1: Initial pH testing 1) First use the wide range pH paper to test the pH of the liquids given. 2) When you test with the wide range paper first (which reads pH from 0-13) be sure
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studying the effect of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure of the
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