Purpose: The purpose of this lab was to test how objects slightly acidic or slightly basic will affect the way catalase‚ the enzyme tested‚ denatures‚ or breaks down. Hypothesis: If the potato is acidic‚ it will react with the H2O2 more than it will with the raw‚ plain potato because the acid will denature the enzyme faster. The manipulated/independent variable is the raw‚ plain potato while the responding/dependent variable is the other types of potatoes used in the experiment. Materials:
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Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL
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Amylopectin The absorbance value(x) read from cuvette containing starch and water represents the total amount of starch-iodine complex. The absorbance value(y) read from cuvette containing starch‚ water and α-amylase at the respective temperature or pH represents the amount of starch-iodine complex which is left after the enzyme has hydrolyzed the starch.In order to get the amount of product;P (maltose and glucose) formed‚ need to subtract the (y) value from the (x) value.
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Billy Kong Chem&163 Lab Report 1 Analysis of Acids‚ Bases‚ pH and Salt Purpose: Explore various methods for determining the characteristics of acid/base solution. The goal of this experiment include the investigation of various acid/base indicators‚ calculating the change of pH with concentration‚ change of pH due to the addition of salt and different between strong and weak acids/bases. Safety and Disposal: Use extreme caution when handling concentrated acids and bases. Dispose of all
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Background An indicator is a halochromic chemical compound that is added to a solution to determine its pH. It is a chemical detector for hydronium ions. The indicator changes colour to show the pH of the solution. Some indicates are not very precise and only tell us whether the solution is acidic or basic. When the indicator is added to the solution‚ they bind to hydrogen or hydroxide ions. The different electron configurations of the bound indicator cause the indicator ’s colour to change.
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at different pHs Abstract These experiments aimed to determine the optimum pH ranges various buffers are effective and provide opportunity for the use of the Henderson-Hasselbalch equation to prepare a buffer of a specific pH. Three different buffer systems were initially investigated; volumes of weak acid and weak bases of specified concentration were prepared and titrated against strong acid or strong base solutions with pH readings taken at frequent intervals to determine pH ranges over which
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CAT - Measuring the pH of common household substances 1. Predict the pH value of substances & estimate the pH value by using the universal indicator. 2. Use the indicator to identify acidic‚ basic‚ and neutral substances found in any home 3. Classify household items as acids and bases 4. Collect organise and analyse results in tables and graphs (if necessary) 5. Analyse results and explain the reason the substances have the pH obtained. Aim – To use a universal
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Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase‚ during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic. This
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Ranking Salt Solutions by pH 6-2: Ranking Salt Solutions by pH In this assignment you will be asked to rank aqueous solutions of acids‚ bases‚ and salts in order of increasing pH. This is most easily done by first identifying the strong acids that have the lowest pH‚ the strong bases that have the highest pH‚ and the neutral solutions that have a pH near 7. The weak acids will have a pH between 1 and 6 and the weak bases between 8 and 14. The exact order of weak acids and
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Joshua McMahon IB Chemistry Matt Chase 3A 2/13/15 Finding the PH of different concentrations of orange juice. Research Question By diluting concentrated orange juice with water‚ can you significantly change the PH value? Introduction Across the world‚ it is thought and widely accepted to believe that Orange juice is the cure to many terrible diseases. However‚ this may not be true anymore. It has been said that Oranges are very high in vitamin C‚ therefore one should drink it every morning
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