June 25‚ 2013 Lab partner: Catalan‚ Christian A. Date Due: July 5‚ 2013 Date Submitted: July 5‚ 2013 EXPERIMENT 1 PREPARING BUFFERS AND BUFFER CAPACITY INTRODUCTION A buffer solution is one in which the pH of the solution is "resistant" to small additions of either a strong acid or strong base. Buffers usually consist of a weak acid and its conjugate base‚ in relatively equal and "large" quantities. A buffer system can be made by mixing a soluble compound
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the titration curves to calculate the percent of the active ingredients in the commercial cleansers. to determine the Ka of a weak acid. Procedure Overview • • • • after calibration of the pH electrode and determination of the flow rate for the automatic titration‚ a NaOH solution is standardized against HCl. a pH titration curve for acetic acid is obtained and its pKa is determined. an unknown sample of Lysol is analyzed for its HCl content using the standardized NaOH. an unknown sample of Liquid
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neutral pH levels around 7. Introduction Enzymes are proteins that catalyze metabolic reactions vital for the survival and functioning of cells [1]. Without enzymes‚ metabolic processes would occur at unfeasible rates. Catalase is a naturally occurring enzyme that breaks down hydrogen peroxide into water and oxygen; it is essential to cellular respiration. I asked if enzyme activity was affected when exposed to different conditions‚ such as temperature‚ substrate concentration‚ and pH levels
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At 20 seconds‚ pH = 7.40 2. At 40 seconds‚ pH = 7.39 3. At 60 seconds‚ pH = 7.38 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? yes‚ it went down by .01 pH 5. Was the pH level always within the “normal” range for the human body? 7.35-7.45 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No Activity 2a: Hyperventilation – Run 1 1. At 20 seconds‚ pH = 7.48 2. At 40 seconds‚ pH = 7.56 3. At
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BioLab3 Lab Report 5 Enzymes Student Name: Cooper Lyon I. Enzyme Structure and Function EXERCISE 1 – Preparation of an enzyme activity standard At five minute intervals over the next fifteen minute period‚ record the color intensity of the solution of each test tube. Time (min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15
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Homework 1_Key BCH2333 Winter 2015 Topics covered: review‚ Chemical Evolution‚ Water‚ Non-covalent bonds‚ Acid/base‚ pH 1. i) Convert using scientific notation to liters: 4 nanoliters‚ 4 x 10-9 L‚ 20 microliters‚ 2x10-5 L‚ 35 milliliters‚ 3.5 x 10-2 L‚ 10 deciliters‚ 1 L‚ 7 kiloliters 7 x 103 L ii) Convert using scientific notation to grams: 6 mg‚ 6 x 10-3 g‚ 21 µg‚ 2.1 x 10-5 g‚ 503 ng‚ 5.03 x 10-7 g‚ 10 kg‚ 1 x 104 g 2. Which of the following properties of water explains its ability to dissolve
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will decrease. Each enzyme functions at an optimum pH. Well according to the graph‚ you see that the enzyme did not function at all when boiled which can be explained by the fact that it got denatured. Denaturing means the secondary and tertiary structure of the enzyme does get disrupted so bonds would definitely be broken. What seemed to be the optimum temperature and pH for the enzyme? In other words‚ at what temperature and pH was the rate of reaction fastest? How did you determine
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acid-base titration can be detected by the use of a pH indicator‚ a pH meter‚ or a conductance meter. The use of a pH meter to quantitatively determine the end point of a titration reaction is preferred to the use of visual indicator. This is because the pH meter monitors the change in pH at the equivalence point rather than just observing the color change in the visual indicator‚ and this eliminates the indicator blank errors. The use of a pH indicator is often regarded as tedious as compared to
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LAB 6. ACIDS AND BASES: PH AND BUFFERS PURPOSE: To determine the pH of common acids and bases using a pH meter‚ pH paper‚ and red cabbage indicator. To test the effect of adding an acid or base to a buffer solution. SAFETY CONCERNS: Always wear safety goggles. Wash with soap and water if skin contacts acids or bases. ACIDS: An Acid is a substance that when dissolved in water will produce hydrogen ions‚ H+‚ in the solution. An acid that does not contain carbon is called an inorganic
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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