in neutral pH. To do this the experiment was carried out so that tubes containing a reaction solution of the Amylase enzyme and starch were simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After concluding the reactions they were placed into a spectrophotometer (A580) for analysis. Graphing the values of the absorbance to time for each pH it was found
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EXPERIMENT A: CIS-TRANS CONVERSION OF BUTENEDIOIC ACID PRE-LAB ASSIGNMENT 1. Maleic acid is the cis version and fumaric acis is the trans version of butenedioic acid. 2. 3. See attached paper. 4. Maleic anhydride is the compound that crystalizes in part A in the experiment. 5. HCl acts as a catalyst to break one of the carbonyl group in the maleic acid which rotates the structure to form a trans structure of the butonal acid which is the fumaric acid. And it does so by donating
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Grab a 40 mL beaker and fill the normal water in the beaker to 40 mL and grab a pH tape then put it into the water. Take the pH paper out of the water and match it to the best similar color and record the data‚ the pH of normal water is 7. Next is to test the conductivity of the water‚ grab the conductivity probe by putting into the water and hit the green arrow. Let the probe be in the water for
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Introduction- Can a filter be made for the polluted water so the water will have a clarity of 0%. If a filter is built for the polluted water‚ then the waters clarity will be 0% and the pH value will be 7. The purpose for this lab is to get the polluted water to a clarity of 0%. Water pollution is playing a major role in today’s ecosystems because the water is becoming unsafe to live in and unsafe to consume. “Water pollution is any physical or chemical change in water that adversely affects organisms”
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Biochem. J. (1995) 305‚ 17-20 (Printed in Great Britain) 17 RESEARCH COMMUNICATION The effect of low temperatures Nicole MORE‚ Roy M. DANIEL* and Helen H. PETACH on enzyme activity Thermophile Research Unit‚ University of Waikato‚ Private Bag 3105‚ Hamilton 2001‚ New Zealand The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain ANI and f‚- enzymes‚ glucosidase from Caldocellum saccharolyticum expressed in Escherichia
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Title Laboratory Techniques and Measurements Purpose The purpose of this experiment is to determine mass‚ length‚ temperature‚ volume‚ and density of objects and liquids using common measuring devices and algebraic formulas. The metric system‚ the most common set of measurements used in science‚ which includes meters‚ liters‚ Celsius‚ and grams will be used. Procedure The following items were used within the experiment: (1) Metric ruler with centimeter (cm) and millimeter (mm)‚ (1)
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Does the amount of time that orange juice is swished in a human mouth affect the pH of the saliva‚ which could inhibit the digestion of the starches in the orange juice? Hypothesis: The longer 2 mL of orange juice is held in a test subject’s mouth‚ the greater the ability of the test subject’s saliva will become to break down starches. Dependent Variable: pH of the saliva. Independent Variable: time that the juice is swished in the mouth. Controlled Variables: Orange juice only. The
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Evaluation of gel A. pH: The pH of the various gel formulations was determined by using digital pH meter. B. Appearance: -The prepared gel bases were inspected visually for clarity‚ colour and presence of any particles. C. Homogeneity: All developed gels were tested for homogeneity by visual inspection after the gels have been set in the container. They were tested for their appearance and presence of any aggregates. D. Skin irritation test: Test for irritation was performed on human volunteers.
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Syllabus Cambridge O Level Biology Syllabus code 5090 For examination in June and November 2011 Note for Exams Officers: Before making Final Entries‚ please check availability of the codes for the components and options in the E3 booklet (titled “Procedures for the Submission of Entries”) relevant to the exam session. Please note that component and option codes are subject to change. Contents Cambridge O Level Biology Syllabus code 5090 1. Introduction ............................
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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