+ Ferric ions à Ferrous sulfide (black precipitate) Phenol red (ph indicator) Alkaline slant/Alkaline butt (K/K) NO FERMENTATION NOT MEMBERS OF ENTEROBACTERIACEAE Stab and Streak GLUCOSE FERMENTATION ONLY Alkaline slant/ Acid butt (K/A) 18-24 hrs incubation Acid slant/ Acid butt (A/A) 8-12 hrs incubation Acid slant/ Acid butt(A/A) 18-24 hrs incubation LACTOSE (SUCROSE) FERMENTATION H2S PRODUCTION Indicators: Sodium thiosulfate Ferrous sulfate (+) black color/black precipitate
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Aim: To carry out a fist hand experiment in the extraction of red cabbage pigment‚ use this extracted pigment to standardize against know pH’s‚ test 5 household items to determine the possible pH of these items by using the standardization and assess the pigment’s ability to be used as a Universal Indicator. Risk Assessment: When dealing with fire‚ the Bunsen burner should always be switched to the safety flame before lighting. There will also be hot water during the experiment‚ which means that
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Titrations are also often used to determine the pH of a solution. This is important because without knowing how acid or basic a solution is people could hurt themselves and others but mixing chemicals together that are lethal. The objective of the is lab is to develop familiarity with the concepts and techniques of titration and to determine the concentration of an acidic acid solution. It was necessary to add Phenolphthalein because it acts as an indicator‚ that is a weak acid‚ so it is easier
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Sterilization by Saturated Steam Mukul Bhatnagar: 21464987 Group Members: Chiew See Hau‚ Daryl Picard‚ Niamh Meagher‚ Musawwir Miriah Introduction Through the course of an entire day‚ an average human will come into contact with millions of different bacterial species. Just as human exposure to these bacterial species is high‚ the utensils we commonly use are subjected to a similar degree of exposure. Generally the temperature required to destroy many species of
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a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface of Simmon’s citrate agar. Followed by overnight incubation at 37°C. Day after the slant was observed for a change
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Ranking Salt Solutions by pH 6-2: Ranking Salt Solutions by pH In this assignment you will be asked to rank aqueous solutions of acids‚ bases‚ and salts in order of increasing pH. This is most easily done by first identifying the strong acids that have the lowest pH‚ the strong bases that have the highest pH‚ and the neutral solutions that have a pH near 7. The weak acids will have a pH between 1 and 6 and the weak bases between 8 and 14. The exact order of weak acids and
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Jobelle J. Department of Chemical Engineering‚ Faculty of Engineering University of Santo Tomas España‚ Manila February 27‚ 2013 Abstract The analyte used is the soda ash which is titrated with an HCl titrant‚ standardized by Na2CO3. The indicators used are phenolphthalein for basicity and methyl orange for acidity. The two volumes of the titrant are then used to calculate percent composition of soda ash analyte. At the end of the experiment‚ the calculated average percent by mass of Na2CO3
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find the pKa of an unknown‚ pH meters are used during titrations to measure the potential difference in a solution by measure the difference of hydrogen-ion activity in a solution and reporting the pH. The pH is then plotted against the volume of solution added which provides the titration curve of a substance. From the titration curve information can be gathered about how many protons were dissociated and the equivalence point. pH=pKa+log([H]/[OH] ) pKa=pH-log([H]/[OH] )
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tested. Data Table 1: Chemicals Provided in Lab Kit. Well # Chemicals Reaction A-1 NaHCO3 and HCl-C02 Remained clear‚ very bubbly‚ almost as if forming a gas‚ which it was. Carbon Dioxide. B-1 HCl and BTB The results of adding the blue indicator was a color change. It turned orange‚ indicating acidity. C-1 NH3 and BTB It remained blue‚ indicating a base. D-1 HCl and blue dye The mixture turned green. E-1 Blue dye and NaOCl Blue at first‚ then when HCL was added to the mix‚ it turned green
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Self-design Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity
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