Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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learning how to measure the pH of several different liquids as well as learning how to change the pH of those liquids by adding other acidic and alkaline mixtures. Materials: Graduated cylinder- used to measure the various liquids 4 100 mL beakers- used to hold liquids when measuring pH pH meter- used to measure the pH of the various liquids Tap water- liquid we are trying to find pH of Distilled water- liquid we are trying to find pH of Soda- liquid we are trying to find pH of Milk- liquid we
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professor! I chose to discuss topic 2 this week. The topic is Discuss how buffers help to regulate body pH. The pH level in the blood is maintained by the kidneys and the lungs. The pH level on the blood should be at 7.4. In order for the kidneys and lungs to maintain this pH level it affects the buffers in the blood. The buffer is a substance that is resistant to the change in the body’s pH level. Basically the buffers can make an acid or base less potent and try and neutralize it so the body is
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College General Biology I Enzymes‚ test for effect of pH on catalase activity Purpose The main purpose of this experiment was to learn about enzymes and how to test for the effect of pH on catalase activity and to be able to tell if a reaction is an exergonic or endergonic process. Introduction Enzymes are made from amino acids‚ which are made from proteins. In order to make an enzyme‚ hundreds of amino acids are strung together in a very specific and unique order and eventually is folded
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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The Effects of Heat on the pH of the Vegetable and Their Pigment PURPOSE The purpose of this experiment is to see how pH levels of certain vegetables change while being cooked in four different mediums (frying‚ boiling‚ steaming‚ roasting)‚ and how the varying pH levels change the coloring of the vegetables. We will analyze how the different method of cooking/heating changes the levels of pH. BACKGROUND INFORMATION This experiment is based around the different pigments in vegetables‚ of which
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The Effect of Starch on the Activity of Amylase with pH Variable Lab Report‚ Fall 2011 East Tennessee State University Department of Biological Sciences By: Shelby Brackett Date Performed: October 10‚ 2011 Lab Instructor: Joseph Kusi Biology 1111‚ Section 018 Abstract Enzymes are very important in chemical reactions. They are used to speed up the reaction taking place. They act by binding to a specific substrate and form an enzyme-substrate complex that may put stress on chemical bonds
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Use Table M and various indicators to determine the pH of acetone Equipment: Test tubes‚ test tube rack‚ acetone‚ various indicators‚ tweezers Procedure: 1. Fill each test tube with a few drops of acetone 2. Put 2 drops of an indicator into 1 of the test tubes 3. Record color change 4. Determine the pH range based on the color change using Table M and record data 5. Repeat for each indicator 6. To test litmus‚ dip red and blue litmus into acetone and determine pH based on color change
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Introduction Background Indicators are used in acid-base titrations to determine end points as both acid and base are colourless in nature. However‚ indicators chosen must be suitable according to the type of reactions such as: - Strong acid-weak base titration - Weak acid-strong base titration - Strong acid-strong base titration - Weak acid-weak base titration Only the first two mentioned above will be investigated in this experiment. Aims/Objectives 1. To understand
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endpoint is the point at which the titration is complete‚ as determined by an indicator (see below). This is ideally the same volume as the equivalence point—the volume of added titrant at which the number of moles of titrant is equal to the number of moles of analyte‚ or some multiple thereof (as in polyprotic acids). In the classic strong acid-strong base titration‚ the endpoint of a titration is the point at which the pH of the reactant is just about equal to 7‚ and often when the solution takes
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