TREATMENTS DESCRIPTION 1 100% concentration of Duranta Leaves extract 2 75% Duranta Leaves extract 3 50% of Duranta Leaves extract 4 Mancozeb Preparation of the materials The materials used in this study were Duranta leaves‚ Fusarium sp.‚ petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves‚ face masks and
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Problem: How do protease in some fruit juices help softening meat? Hypothesis: The protease in some fresh juices digests collagen‚ which gives meat a tough texture by holding meat fibres together. Design of investigation: In order to test the hypothesis‚ the presence of protease in those fresh juices was tested at first. Then the amount of them was needed to be compared for finding their difference in effectiveness in tenderizing meat. Control experiments were set up for proving protease was
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This experiment tested the growth of E.coli with inserted plasmid on an agar plate with Ampicillin. One colony of E.coli resistant to Ampicillin was grown during this experiment. The overall goal of the experiment was to successfully grow E.coli on the agar plate‚ which would show that the plasmid had been effectively inserted into the bacteria’s genes. This experiment helped students understand how plasmids were inserted into bacteria and used in real life situations. It also showed how the bacteria’s
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Your Full Name: Lauren Sinay UMUC Biology 102/103 Lab 1: Introduction to Science INSTRUCTIONS: On your own and without assistance‚ complete this Lab 1 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). To conduct your laboratory exercises‚ use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments
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2013-2014 ABSTRACT The effect of molecular weight on the rate of diffusion was assessed using the agar-water gel test. The agar-water gel set up was composed of a six petri dish of agar-water gel containing two wells. Pinch of potassium permanganate (KMnO4) and methylene blue (C16H18N3SCl) were simultaneously introduced to each dish and measured the distance and its rate. Methylene blue‚ having the largest molecular weight‚ displayed the smallest diameter 15.67 and diffused at the slowest rate (0
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The Effect of Molecular Weight on the Rate of Diffusion of Potassium permanganate (KMNO4)‚ Potassium dichromate (K2Cr2O7) and Methylene blue ¹ ABSTRACT The effect of molecular weight on the rate of diffusion was determined using the Agar-water Gel Test. One drop of potassium permanganate (KMNO4)‚ potassium dichromate (K2Cr2O7) and methylene blue were used. Each substance has a respective molecular weight of 158 g/mol‚ 294g/mol and 374 g/mol. The diameters (in millimeter) were measured
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Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Garlic Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar
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Serial Dilution Activity Many applications require the determination of microbial numbers. Those applications can be either clinical or in a research setting. Clinical applications include determination of antibiotic efficacy and as well as therapy. Research applications include determination of the effectiveness of antimicrobial chemicals‚ radiation‚ etc. The viable count is most common or standard method used to quantitate bacteria. With this method only microbes that are alive and able to reproduce
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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