carefully transfer onto 4 petri dishes at a tilted angle‚ with the petri dish lids at an angle as well to reduce airborne contamination. After the end of each pour—no more than 2/3 of the petri dish’s capacity—the flask containing the liquid media was sterilized briefly through the Bunsen-burner by running it through the flames a couple of times for a few seconds‚ then the aluminum foil was also briefly sterilized by this same method. These lids were closed on the petri dish and left
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INTODUCTORY PLANT PATHOLOGY C123P1 Isolation & Study of Sclerotinia (Monilinia) Fructigena Extraction of Polygalacturonase(PG) enzyme Assay Sclerotinia (Monilinia) Fructigena • 10g of infected tissue was taken and ground in a mortar and pestle with 100Mm PH5 citrate buffer then filtered through 4 layers of muslin. • Half of this mixture was boiled at 50°C for 10 minutes and the other half was left at room temperature. • The above steps were repeated with a sample with healthy
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Materials: The materials we used in this lab were a petri dish with agar‚ forceps‚ water‚ alcohol‚ hydrogen peroxide‚ antibacterial soap‚ filter paper disc‚ tape‚ and a permanent marker. Procedure: We started this lab on May 12‚ 2014. Using a permanent marker on the bottom of the dish‚ we divided it into 4 sections. We labeled the sections‚ W (water)‚ A (alcohol)‚ S (soap)‚ and HP (hydrogen peroxide). Then‚ we labeled the perimeter of our dish with our initials and class period. Kyra rubbed her
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How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory | | | | | | | | | |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel |
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water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution (pH of a 11) *100mL beaker (3 x) *Bunsen burner *lighter *heat-proof mat *tripod *gauze mat *stirring rod *Petri dishes (6x) *small wooden blocks *fine black marker Agar Plate Inoculation: *fine black marker *ruler *sticky tape *sterilized cotton bud (6x) *paper towels *meat extract stock *pipette dropper *50mL conical flask Method: Agar
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characteristics could be distinguished different colonies‚ such as‚ shape‚ size‚ color‚ and consistency. 4. Why should a Petri dish not be left open for any extended period of time? Ans: A Petri dish is a medium which using to grow bacteria cultures. If we leave the plate open on the air‚ it might increase the probability that microbes culture we don’t expect growing in the Petri dish. 5. Why does the streaking method you used to inoculate your plates result in isolated colonies? Ans: we streak
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COMPARISON BETWEEN PLACENTA AGAR AND NUTRIENT AGAR ON GROWTH OF Staphylococcus aureus AND Escherichia coli Chloe Dominique Acero Kristine Marie Gonzales Hannah Marie Hermosisima Patrisha joy Morales Joanna Keziah Ramos Group 4 BSMT3E Background of the Study Placenta is an organ characteristic of a true mammal during pregnancy‚ joining mother and offspring‚ providing endocrine secretion and selective exchange of soluble‚ but not particulate
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such as differences in light intensity or dampness. * A lamp‚ acting as the light source was placed 20cm from the choice chamber and remained constant throughout the experiment. * Two petri dishes can be cut and glued together; another two petri dishes were placed upside down to cover the first two petri dishes‚ making a simple choice chamber. * There is a problem with the woodlice clumping up with each other (this is one of the things they naturally do when the get close to something or
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002141-0012 Nuba Jackson IB Biology Microbiology IA How effective is Lysol in the reduction of bacterial growth compared to Pinesol in reduction of E. Coli growth in agar at room temperature? Background Information: Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99.9 percent of bacteria. Lysol contains
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separately [19‚21‚25‚27‚34]. Nutrient agar was prepared‚ then sterilized in an autoclave and poured in sterile Petri plates. After cooling of nutrient ager in Petri plates‚ the studied organisms were grown on agar. After that‚ the sterili Paper discs of Whatman saturated with solution of investigated compounds were placed in the agar by working holes using a sterile crook borer. These Petri dishes were incubated for 24 h at 37oC [25]. The standard antibacterial drug gentamycin was screened under similar
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