to ensure that Drosophila melanogaster fed from only one point and to control evaporation of our solutions. Petri dish samples were replicated 7 times including the control. Petri dishes were imaged immediately (Cano Scan 4400F) and Drosophila melanogaster (25) were placed in each dish. For our control‚ a petri dish containing no Drosophila melanogaster was made (Sellier et al.‚ 2010). Petri dishes were sealed and covered with tin foil. This was done to create darkness and ensure that Drosophila melanogaster
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reproductive spore. Reproductive Spore = a means of both reproduction and dissemination of molds‚ since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide
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cotton swab METHOD: 1. Prior to placing any food products into the petri dish‚ categorize them into the control (agar plate containing no food products) and the experimental (agar plates containing food products) via labelling. 2. Leave petri dishes inverted for a time period of 48 hours as to provide a clear illustration as to the absence of microbes and forms of contamination on the agar surface. 3. Before handling the petri dishes‚ equip 1 pair of sterile gloves using the sterile glove technique
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the testtube then plug it with cotton. 4) Grab the inverted plated media and flame sterilize the mouth. 5) Do a multiple streaking in the plated media with your cotton swab. 6) After streaking‚ flame sterilize again the mouth of the petri dish and then invert it. 7) Throw the cotton swab in a plastic with diluted Lysol. Results and Observation: Broth: Source: Toilet bowl (girls)
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Plant Tissue Culture 151 Chapter 9 Plant Tissue Culture Techniques Lorraine Mineo Department of Biology Lafayette College Easton‚ Pennsylvania 18042 Lorraine Buzas Mineo (B.S.‚ Muhlenberg College; M.A.‚ Duke University) is a lecturer in the Department of Biology‚ Lafayette College‚ and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include
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energy in green plants. The objective of this experiment is to measure the rate of photosynthesis Hypothesis: The petri dish that is exposed to the most light and with the NaHCO3 solution will have the best results and the petri dish that is kept in the dark will have the poorest results. Material and Methods: 1. Get 4 deep petri dishes and label them 1‚ 2‚ 3‚ and 4. Fill dish 1 about 2/3 full with distilled water. Fill the other 3 about 2/3 full with 0.2% NaHCO3 solution. Set these aside for
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The affect of temperature on the rate of movement of woodlice Aim: The aim of this experiment is to establish whether a change in temperature affects the rate in which woodlouse move. I measured the rate by timing the woodlouse to move a set distance of 20cm‚ and altered the surrounding temperature by submerging a clear tube in water with different temperatures controlled by a water bath. Research: It is to be believed there over 3000 different species of woodlice‚ a total of 42 species
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Aim: To investigate the effect to the potato cells in the different solute concentration water Introduction; Water can move through the different cells due to the difference of water potentials in the cells. If there is a higher solute concentration in the cell than outside the cell‚ the water will move into the cell. However‚ if the concentration of inside the cell is lower than the outside‚ water will not move into the cell. This process is called osmosis. Research question; This investigation
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marcescens. Sterile petri dishes Liquid nutrient agar Sterile filter paper disc PROCEDURE i. The bottom of a petri dish has been labeled with the name of the organism‚ the chemical agent and name and date of experimenter and experiment respectively. ii. One or two loopfuls of the organism has been taken from the cultures provided and a tube of liquid nutrient agar has been aseptically inoculated at 50oC. The mixture has been poured into a sterile petri dish. iii. When
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second agar plate‚ open the “stick” end of the sterile cotton swabs to avoid contamination of the swab. Deep the swab in to the sterile water and collect a dust form the corner of the table by swab and rub the swab over the entire surface of the Petri dish without going back over areas you have already swabbed. 3. For the third agar plate‚ divide the plate in two different parts like washed and unwashed finger tip and swipe on each side of plate‚ see the difference between them. 4. For the forth
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