components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by gentle pipetting. The 2X rapid ligation buffer is used in this experiment to saving the ligation time. This ligation buffer contains ATP. ATP is used to catalyze the formation of phosphodiester bond and the reaction of restriction enzyme buffer. The recommended time and temperature of incubation when using 2X rapid ligation buffer are one hour at room temperature (24°C) and overnight at 4°C. In this overnight incubation
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Ebanks v. New York City Transit Authority 70 N.Y.2d 621‚ 518 N.Y.S 2d 776‚ Web 1987 N.Y Lexis 17294 United States Court of Appeals of New York Facts: * Julius Ebanks’s left foot got caught in a 2-inch gap between the escalator step and the side wall of the escalator‚ which was owned and operated by the New York City Transit Authority. * He was thrown violently to the ground after reaching the top. His hip was fractured along with other serious injuries. * The standard gap of the
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When a population grows rapidly‚ it can put great stress on an area such as a community or a country. When sudden extreme population growth occurs‚ food will become a prized possession‚ more people will die due to disease and war‚ and the economy will be affected. As a population grows‚ all the additional people will need food immediately to survive. There will not be time to develop new sources of food over many years. Instead‚ more and more people will need food more and more and the food will
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“Rapid technological change has shaped the world we live in today…” The impact of new technology especially in the developed world is evident all around us in the way we communicate and understand the world. There has been significant progress in many spheres‚ such as health‚ engineering‚ education and many others with the technological progress. Sometimes it is far away from positive influence many areas of our everyday life depend on computers and mobile devices and it distanced us from what
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The U.S. Supreme Court case 469 U.S. 528 (1985)‚ Garcia v. San Antonio Metropolitan Transit Authority‚ was first argued on Monday‚ March 19‚ 1984 and reargued on Monday‚ October 1‚ 1984. It was finally decided on Tuesday‚ February 19‚ 1985. The panel consisted of Chief Justice‚ Warren E. Burger and Associate Justices‚ William J. Brennan‚ Jr.‚ Byron White‚ Thurgood Marshall‚ Harry Blackmun‚ Lewis F. Powell‚ Jr.‚ William Rehnquist‚ John Paul Stevens‚ Sandra Day O’Connor. The majority vote of 5 to
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Rapid and Continuous Change – A modern Perspective By Nathan Jennison As famously held by Charles Darwin‚ “it is not the strongest of the species that survives‚ nor the most intelligent that survives. It is the one that is the most adaptable to change” (Cope‚ 2009 p; 26). Hence‚ in today’s global and dynamic environment marked with hyper-competitive and volatile markets it is widely recognised that an organisation’s ability to manage change quickly‚ productively and positively is a critical
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CHAPTER 1 THE PROBLEMS AND ITS BAKGROUND The rapid growth of computers in field of technology is truly remarkable. Computerized software is considered as assets in any working environment. Now we can see computers everywhere‚ in school‚ different establishment and also is a field of business. Even doctors‚ engineers and accountant use computers in their daily routine. It is because computes made the work a lot easier and faster. These are noted for its capacity to store large amount of information
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International Labour Organization International Programme on the Elimination of Child Labour (IPEC) Investigating the Worst Forms of Child Labour No. 17 Lebanon: Child Labour on Tobacco Plantations: A Rapid Assessment By Consultation and Research Institute May 2002‚ Geneva ISBN: 92-2-112973-X Preface Unacceptable forms of exploitation of children at work exist and persist‚ but they are particularly difficult to research due to their hidden‚ sometimes illegal or even criminal
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Proceedings of COBEM 2009 Copyright © 2009 by ABCM 20th International Congress of Mechanical Engineering November 15-20‚ 2009‚ Gramado‚ RS‚ Brazil MINIATURISED CYLINDER HEAD PRODUCTION BY RAPID PROTOTYPING MELO‚ Rodrigo N.‚ a42144@alunos.uminho.pt Universidade do Minho‚ Campus de Azurem‚ 4800-085 Guimarães‚ Portugal. MONTEIRO‚ António A.C.‚ cmonteiro@dem.uminho.pt Universidade do Minho‚ Campus de Azurem‚ 4800-085 Guimarães‚ Portugal MARTINS‚ Jorge J.G.‚ jmartins@dem.uminho.pt Universidade
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Racca and Edward C. Ratledge(2004) with a title of Factors That Affect and/or Can Alter Mode Choice. Racca and Ratledge(2004) cited from Fang Zhao(2002)which is a members of National Center for Transit Research(CTR) entitled “FSUTMS: Mode Choice Modelling: Factors Affecting Transit Use and Access” included that there is an identification of many of the factors that have been used in modelling. As many studies concerned with the factor affecting mode choice for the journey to work‚ there
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