Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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of farUV: 180-240nm. 1. Near UV CD: 240n-320nm‚ Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography Substances present in a mixture are allowed to distribute themselves between two phases: the stationary phase (fixed) and the mobile phase. As the mobile phase flows over the stationary phase‚ components of the mixture experience many transfers
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Decolorization of Crystal violet dye by Aeromonas hydrophila isolated from textile wastewater Sujata Mani and Ram Naresh Bharagava* Laboratory of Bioremediation and Metagenomics Research (LBMR) Department of Environmental Microbiology (DEM)‚ School for Environmental Sciences (SES) Babasaheb Bhimrao Ambedkar University (A Central University) Vidya Vihar‚ Raebareli Road‚ Lucknow - 226 025 (U.P.)‚ India *Corresponding Author: Dr. R. N. Bharagava Tel.: (+91) 522-2998718; Fax: (+91)
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Mazarei Food Microbiology April 22‚ 2011 Purpose: To test the pH level of many common household object by use of the juice from a red cabbage. Background: pH is the measure of the acidity or basicity of a solution. The pH scale ranges from 1 to 14 with 1 through 6 being acidic and 8 through 14 being basic; 7 is considered neutral. The use of the red cabbage‚ or red cabbage juice is because red cabbage can be used as an acid/base indicator. The pH level of foods is important to food microbiology
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Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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OCEAN FOOD CHAIN ich are not relevant to a general audience: “From the time I was small‚ I have been very active in defending our environment‚” “From the first time I saw environmental protestors‚ I realized that they were all crazy.” Note that some of these topics could be converted into theses that would be arguable to a general audience if they were de-personalized and established as arguable issues. Also keep in mind that personal examples may play a significant supporting role in your
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The kermes dye was considered to be native to the old world‚ “Used by the Aztecs for dyeing and painting” (Douma‚ 2008)‚ and the Incas‚ who loved their reds. Throughout this time‚ various native american groups throughout Mesoamerica and South America used as dyestuff. It wasn’t until the 16th Century‚ that the Spanish conquest pushed the kermes dye into the hands of the Europeans. In fact‚ “When Spanish explorers discovered [the dye] and Conquistadors invaded Central and South America in the late
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Marketing II – Group Assignment Waters Chromatography Division: US field Sales Submitted by: Group 5‚Section D ArkayanBagchi (2010PGP063) Harveer Singh (2010PGP449) Kaushlendra Singh (2010PGP150) Krishna Kishore Burugula (2010PGP163) Loveneesh Solanki (2010PGP170) RohanWagh (2010PGP311) Sameer Morey (2010PGP325) Protagonist The cases does not give any specific issues to be solved by any
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