The reaction between alpha-amino acid and ninhydrin involved in the development of color are described by the following five mechanistic steps: alpha-amino acid + ninhydrin ---> reduced ninhydrin + alpha-amino acid + H2O alpha-amino acid + H2O ---> alpha-keto acid +NH3 alpha-keto acid + NH3 ---> aldehyde + CO2 Step (1) is an oxidative deamination reaction that removes two hydrogen from the alpha-amino acid to yield an alpha-imino acid. Simultaneously‚ the original ninhydrin is reduced
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of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96‚ and the pKa’s found experimentally were 2.0‚ 4.0‚ and 9.85. The accepted pKa values were found to be 2.10‚ 4.07‚ and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid‚ hydrochloride
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FLEXIBLY DIVIDED DOUBLE LUMEN ENDOTRACHEAL TUBE Viren Sunil Kalsekar‚ Joy Lee‚ Jenny Qiu‚ Nicholas Ragosta‚ Javier Ramos‚ David Taylor Massachusetts Institute of Technology Cambridge‚ MA‚ USA ABSTRACT Endotracheal tubes (ETT)‚ are used to maintain patient airway access during surgical procedures. The design presented here addresses a current need of the gold standard ETT by allowing for the passage of a larger bronchoscope through the inner lumen of the ETT while keeping outer dimensions constant
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identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly every physiological event in the cell. Since all amino acids contain at least one amino and one carboxyl group‚ they are classified as amphoteric substances (meaning that they can act as either an acid or as a base) (1). Treating the zwitterion with acid will result an addition
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for Intact proteins and protein hydrlysates (at least tripeptide‚ 2 peptide bonds Result Pink to violet blue color Ninhyrin Test 1‚2‚3-indanetrione monohydrate or triketohydintene hydrate‚ ethanol Alpha- amino group (usually a general test for amino acids) Xanthoproteic Test Conc. HNO3‚ conc. NaOH For W‚F‚Y (aromatic except for H) Blue to blue-violet Oxidative decarboxylation color & deamination followed by (proline:hydroxypr condensation oline gives a yellow
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The Small Intestine Anatomy and Physiology I 3/10/2014 The majority of the digestive process is done by the small intestine‚ absorbing almost all of the nutrients you receive from foods into your bloodstream. The small intestine begins at the pyloric sphincter of the stomach and coils around the abdominal cavity‚ and then opens into the large intestine. “The intestines process about 2.5 gallons of food‚ liquids and bodily waste every day.” (Inner Body) There are three features that the small
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Large Intestine By Pamela Hankinson For HAP II October Large Intestine Many people often confuse the large intestine with the small intestine. However the large intestine is wider (about 3 inches) yet shorter than the small intestine (in humans about 4.9 feet in length as compared with 22 - 25 feet for the small intestine) and has a smooth inner wall. (Britannica) The large intestine consists of the cecum‚ ascending‚ transverse‚ descending and sigmoid colon‚ rectum
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Lumen naturalis: intuition‚ habitus and imagination What is it that sometimes allows the ’question’ that is posed through a musical challenge - a new compositional idea‚ a new musical instrument to master‚ a new improvisational situation - what in these moments is it that sometimes makes the answer emerge with the question‚ in a way similar to how Descartes conceived of the fundamentals of scientific knowing? Just like to Descartes who would refer to it in terms of lumen naturalis (natural light)
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EXPERIMENT 13 DIGESTION IN THE SMALL INTESTINES ------------------------------------------------- ABSTRACT The small intestine serves as the site of major digestive and absorptive processes. In this experiment‚ the action of pancreatic enzymes on representative samples of each food group under different conditions‚ such as increased/decreased pH and presence of other substances‚ were observed. A pancreatin solution was first prepared from a hog pancreas and was completely neutralized using
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range of values that were obtained throughout the whole experiment (Table 10). 5.2. Proximate composition and amino acid analyses
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