Biology Planning Exercise June ‘04 The Uptake of Glucose in Yeast Cells Glucose is absorbed across the cell surface membrane (plasma membrane) of most cells. A convenient way to investigate this is to use a solution of glucose and a suspension of yeast cells. The amount of glucose taken up from the glucose solution by yeast cells in a fixed length of time can be measured. At the end of the fixed length of time‚ further uptake of glucose is prevented by transferring the yeast suspension to
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2.3. Membranes and modules for forward osmosis: 2.3.1. FO Membranes Generally‚ any dense‚ non-porous‚ selectively permeable material can be used as a membrane for FO. Such membranes have been tested (in flat sheet and capillary configurations) in the past for various applications of FO. In early studies‚ the researchers applied various FO membrane materials‚ including bladders of pigs‚ cattle‚ and fish; collodion (nitrocellulose); rubber; porcelain; and goldbeaters’ skin [36]. By 1960‚ Loeb and
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Variables in Glucose Tolerance lab was to examine the changes in blood sugar during glucose tolerance test conducted with test rats. We will be using three rats and perform the Oral Glucose Tolerance Test to measure the glucose levels in the rats blood. The oral glucose tolerance test is a laboratory method to check how the body breaks down (metabolizes) blood sugar. Glucose is the sugar that the body uses for energy. Patients with untreated diabetes have high blood glucose levels. Glucose tolerance
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Partners: Sunniva & Bayu Determining the concentration of glucose of 7UP Glucose solutions prepared as follows:- In each case 250.0 cm3 volumetric flasks used Concentration (%) Mass/g 4.00 10.00 8.00 20.00 12.0 30.00 16.0 40.00 20.0 50.00 Table: 1- Recording of concentration‚ volume‚ and qualitative observations of various solute/solvent/solution used during the experiment. Name Concentration (%) Volume (ml) Qualitative Observations Glucose (C6H12O6) 5 different types: 4%‚8%‚12%‚16%‚20% 5.0 ± 0.1
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OSMOSIS LAB BACKGROUND INFORMATION. Dialysis tubing is being used to model a plasma membrane. Isotonic solutions are solutions that have the same concentration of solutes on both sides of the membrane. Hypertonic solutions are solutions that have more solute to the one with less solute. Hypotonic solution are solutions that have less solute to the one with more solute. RESEARCH QUESTION:which dialysis tubing will gain the distilled water and which one will loss its content
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Introduction In Diffusion‚ Dialysis & Osmosis lab‚ we discovered that we were going to observe and understand the conditions under which diffusion‚ dialysis‚ and osmosis occur. Then we constructed models of diffusion‚ dialysis‚ and osmosis. We predicted that the direction of change would be from the membrane to the beaker. We understood how these processes affected the selectivity of the cell membrane. The second part of the lab was dealing with dialysis. In this part we studied starch and sodium
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Objective: To observe the effects of osmosis on eggs placed in vinegar (5% acetic acid)‚ syrup (60% sugar solution)‚ and water (100% distilled). Hypothesis: Hypothesis #1: It was hypothesised that the egg would gain 59 grams of mass after soaking in vinegar for 24 hours. Hypothesis #2: It was hypothesised that the egg would become larger when soaked in syrup than it once was due to the hypotonic environment. Hypothesis #3: It was hypothesised that the egg would soak in water for 72 hours‚ losing
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Introduction: Glucose is one of the few chemical constituents of the blood that can change noticeably in its concentration. Many diseases cause a change in glucose metabolism but the most frequent cause of an increase in blood glucose is diabetes mellitus. The early detection of diabetes is important to avoid the many complications of the disease. The glucose tolerance test commonly done by recommended level for adults of a 75 g glucose drink to be consumed within a 5 min time period. The timing
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aspects of metabolism of other inorganic compounds have been studied in some detail‚ predominantly in the yeast‚ Saccharomyces cerevisiae. Table 3-1: Nutrients for growth of yeast (S. cerevisiae) cells. Substrate Saccharose Maltose Melibiose Glucose Ethanol Lactate Glycerol Intermediates Enzymes Invertase Maltase Melibiase Acetaldehayde > Acetyl-CoA> Oxaloacetate> Pyruvate> Glycerol-3phosphate> Dihydroxyacetonphosphate
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Demonstrating osmosis Introduction The movement of water in and out of a system bounded by a membrane permeable to water‚ but not certain other substances‚ can be inferred by either mass changes or volume changes in that system. It is possible to monitor other changes in physical conditions‚ such as texture. Monitoring mass enables quantification of the changes‚ whereas other changes may only be qualitatively measured. Equipment / materials 4 eggs Sugar Distilled water Acetic acid Scales
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