chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop the effects of catechol oxidase on potato cells by testing the change in absorbency over time. Our data supported our hypothesis that EDTA would have a greater change in absorbency over time than PTU. Environmental Parameters of Enzyme Activity Introduction In this experiment‚ we used potato cells (CO) to act as
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reaction. Therefore‚ it will be presumed that the result of this experiment by isolating the polyphenol oxidase within the potato extract through the oxidation of 5mM dopa solution with highly concentrated enzymatic preparation of polyphenol oxidase with a buffer as the diluent will indicate a rapid increase in the rate of the reaction as well as the amount of activity present in the potato extract. Proposing that Vmax is proportional to enzyme concentration‚ a linear relationship should be present and enzymatic
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demonstrate the process of osmosis using potatoes‚ and to determine the concentration of the cytoplasm of the potato. To demonstrate this‚ we had 6 test tubes and poured in different sucrose solutions of the following concentrations: 0.0M‚ 0.1M‚ 0.2M‚ 0.5M‚ and 1.0M. Once added in with all the sucrose solutions‚ we added in the 6 cylindrical potatoes of equal diameter and length. Before and after putting it in the potatoes‚ we had to weigh the initial and the final mass of each potato. After you have left
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Synthesis Procedure 1 -1.0 Introduction A Cobalt-Amine-Halide compound is synthesized from cobalt (II) chloride hexahydrate. An orange-tinted solid is produced and is considered to be unknown since the specific ligand amounts are unknown. By determining the percent composition of various elements and compounds in the unknown‚ its true identity can be predicted. Chloride‚ ammonia‚ and cobalt are three examples of percent compositions determined to help narrow the selection of possible unknowns
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Physics Lab Report 1. For the wavelength measurement of different colors in the Hydrogen spectrum done in the lab‚ tabulate your data recorded along with the wavelength calculations performed for all colors in the spectrum. (2 points) Line Color a_left (m) a_right (m) a_average (m) sinq nm Red 0.235 0.27 0.2525 0.182145 5.47E-09 Green-Blue 0.17 0.33 0.25 0.180505 5.42E-09 Indigo 0.16 0.35 0.255 0.18378 5.52E-09 Violet? 0 0 0 0 0 To find the wavelength for all of the colors in this lab we used two
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aspects that need to be considered during the experiment are to provide caution whilst handling benedict’s solution as it can be caustic to the skin and eyes. As a safety measure wear a lab coat‚ gloves and protective goggles at all times . Handle glass ware with care to minimise breakage. Follow general lab practice. METHOD The apparatus was
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Enzyme Lab Report Introduction: Enzymes are proteins that enable chemical reactions. In the enzyme lab‚ the effects of concentration‚ temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction. Materials and Methods: Experiment 1‚ 2‚ & 3 Experiment 1 examined the effects of concentration on catalase activity. Experiment 2 examined the effects of concentration in temperature
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CardioLab Report Full Name || Date | 01/02/2011 | Use this document to report your findings from the CardioLab Exploration Experiment. The lab report consists of three sections: Data‚ Exploration‚ and Lab Summary. * Data: copy any data‚ graphs‚ charts‚ or notes that you have saved in your CardioLab online notebook into this section. * Exploration: Answer the questions. The questions in the Exploration section are the same questions in your CardioLab instructions. * Lab Summary:
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2013 Lab Day/TA/Group: Wednesday(1:35pm)/Group A Labs reports must be typed and chemical structures must be drawn with ChemDraw. Report must not exceed three pages (including this page). Page limit does not include any attached spectra or references. ** Deductions for hand written report/structures and exceeding page limit ** Report Breakdown Data/Results: _________________ /10 Discussion: _________________ /10 Report Total: _________________ /20 Other Lab Marks
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inserted into the spectrophotometer and measured with the 420 nm wavelength. This procedure was repeated for the remaining test tubes. Exercise B required 4 test tubes labeled with “A‚B‚C‚ or D” and they held the solution of different pH buffer and potato juice volumes‚ and 1 mL of water (Table 4). These tubes were the blanks for the spectrophotometer for recalibrating it. The additional 4 experimental test tubes were composed of the same contents but 1 mL of the substrate catechol was added instead
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