University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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BioLab3 Lab Report 5 Enzymes Student Name: Cooper Lyon I. Enzyme Structure and Function EXERCISE 1 – Preparation of an enzyme activity standard At five minute intervals over the next fifteen minute period‚ record the color intensity of the solution of each test tube. Time (min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15
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Grade 11 physics: SPH3U1-01 | Physics Lab Report | Specific Heat Capacity of Brass | | Jin Jin Shi | 2012/12/6 | Instructor: Mr. Nailer | I. Introduction The amount of heat required to raise the temperature of a solid body depends on its change in temperature (ΔT)‚ its mass (m)‚ and an intrinsic characteristic of the material forming the body called specific heat (cp). The heat is calculated from the equation II. Purpose The purpose of this laboratory is to determine
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perceived performance in which plants and other defined organisms use the energy of photons to convert carbon dioxide and water into a simple monosaccharide sugar known as glucose. Photosynthesis provides the fundamental energy source for essentially all living organisms. The most substantial and valuable byproduct of photosynthesis is oxygen‚ one of the most abundant elements living organisms depend on. Photosynthesis occurs in many organisms such as green plants‚ algae‚ various bacterium
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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Different Life Stages of Leaves Introduction Stomata control a tradeoff for the plant: they allow carbon dioxide in‚ but they also let precious water escape. A plant that could get enough carbon dioxide with fewer stomata would have an advantage since it would be better able to conserve its water. Levels of carbon dioxide in Earth’s atmosphere change over time — so at times when the atmosphere is carbon-dioxide-rich‚ plants can get away with having fewer stomata since each individual stoma will be able
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Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor
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absorbance. The importance of the existence of iron in this reaction is that the peroxidases in plant cells respond to environmental stress by‚ in this case‚ catalyzing the conversion of toxic metabolic wastes‚ H2O2‚ into the harmless products‚ H2O and O2‚ faster. Therefore‚ as peroxidases help minimize and eliminate harmful chemicals‚ the iron cofactors that help peroxidase’s activity raise the possibility of plants’ survival in harsh environments.
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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