and differential centrifugation Introduction Mitochondria are important cellular organelles located in the cytosol of cells and is believed to have originated through an endosymbiotic relationship. The unique double layered membrane structure is responsible for the production of the primary energy currency of the cell; adenosine triphosphate or ATP. Small amounts of ATP that the cell can immediately use as an energy source are available during glycolysis of glucose to pyruvate‚ but the amount of ATP
Premium Adenosine triphosphate Metabolism Cellular respiration
Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
Premium Chemistry Concentration Solution
The hypothesis for this lab was that Core B will be the least flexible due to a loss in water. Unfortunately‚ my results led my hypothesis to be incorrect. The results that were received was that Core C was the most flexible‚ Core B was somewhat flexible‚ and Core A was not flexible and stiff. Core A was the most non-adjustable due to it being hypertonic which means that the total concentration of all the dissolved solute particles is greater than the other solution. Core B was isotonic meaning
Premium Water Chemistry Concentration
to pull other substances along with them through a cell membrane. A carrier protein situated in the cell membrane serves as an attachment point for the co-transport of the sodium ion as well as the other substance. Once attached‚ both the sodium ion and the other substance are transported to the interior of the cell together. This is due to the diffusion energy gradient of the sodium ion. Examples of co-transport: Sodium-glucose co-transport mechanism The sodium ion and glucose both attach to
Premium Muscle Classical mechanics Physical exercise
Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
Premium Cellular respiration Adenosine triphosphate Citric acid cycle
Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
Premium Leaf Color Chlorophyll
William Perez Cell Biology 2440 Lab on protein Myosin Proteins are chains of amino acids that perform the most important functions in living organism. Every protein will contain an amino group‚ carboxyl group‚ a different R group and an alpha carbon with two hydrogens. There are nine types of functions proteins can have‚ enzymes‚ motor‚ receptor‚ structural‚ storage‚ transport‚ signaling‚ and special purpose proteins(antibodies). There are four levels of protein structure‚ primary‚ secondary
Premium Protein Amino acid DNA
Sexton December 4‚ 2014 Bot 121 Lab Coleus Lab Report 1. A cutting can form an entire new plant if cutting being used has a meristem. If the cutting has no meristem‚ mitosis will not occur and you will end up with a dead piece of plant material. In order to successfully grow a new plant through a cutting‚ your plant must have adventitious root growth which in order to deliver water and nutrients to the plant. Adventitious roots are created through cell potency where cells differentiate. The hormone auxin
Premium Plant propagation Plant Seed
Mallory Charland February 9‚ 2015 Mutations of E. coli Lab Report Advanced Biology Deducing Mutations of the Lac Operon of E. coli Abstract: In this lab we determined a possible mutation in unique bacterial strains of E. coli by observing the proper responses of wild type E. coli Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes‚ each containing a different substance (lactose‚ glucose‚ water and lactose and glucose)
Premium Transformation Bacterial conjugation Escherichia coli
City University of Hong Kong Dept. of Physics & Materials Science AP2104 Mechanics of Solids Laboratory Manual Experiment 1 Pure Bending of a Beam Experiment 2 Torsional Deformations Experiment 3 Yield Criteria for Ductile Materials under Plane Stresses Experiment 1 Pure Bending of a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction
Premium Force Torque Shear stress