To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or cream-colored
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Wanni Lin Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are
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Stephen White Biology Lab 11.1 Observations of the spicules of a sponge Supplies Microscope Prepared slide: Sponge Lab notebook Colored pencils Natural Sponges Hypothesis We will learn more in depth about sponges and the complexity of this animal Procedure Set up microscop as instructed in previous expiraments. Place the prepared slide under the microscope. Obeserve under low power and draw what you see in your notebook. This slide shows you the spicules‚ wich make
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Objective The purpose of this lab is to determine the particle size distribution of the fine and coarse aggregates by sieving. Equipment and Material Balance‚ sensitive to within 0.1% of the weight of the sample to be tested Standard sieves for grading of fine aggregates- 4.75 mm‚ 2.36 mm‚ 1.18 mm‚ 300m‚ 150m (# 4‚ 8‚ 16‚ 50 and 100) Standard sieves for grading coarse aggregates- 1 ½ in.‚ 1 in.‚ ¾ in.‚ ½ ‚ 3/8 in.‚ 4 in‚ plus a 4.75 mm(#4 sieve) Fine (0.5 Kg) and coarse (2 to 20 Kg depending
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The objective of this lab was to recreate the color profile of a given solution. In this case‚ the solution was Powerade. The final solution should match the absorbance values at the peak wavelengths (420nm and 628nm) in Powerade. This lab was done using deionized water‚ FD&C Blue #1‚ FD&C Yellow #5‚ FD&C Red #40‚ and a spectrometer. To obtain the correct color profile‚ FD&C Blue #1 and FD&C Yellow #5 were utilized in the sample solutions. The experiment was conducted over two days; the first day
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Abstract Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water‚ PO4 buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate
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This lab activity will go over environmental factors that affect the folded structure of proteins. We used a raw egg and noted the changes that happen when there were changes in temperature‚ pH levels‚ and salinity. Five eggs were cracked open and placed into different bowls with different solutions placed into each bowl. What we looked at was the process of denaturing of proteins seeing how it affected the composition of the raw egg between the yoke and white portion. My hypothesis for this experiment
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The purpose of the lab is to identify the compound based on a constant composition by performing a serious of tests. The hydrate is solid crystals compound and appears to be dry: since an ionic compound (salt) is crystallized from an aqueous solution (water)‚ by heating the hydrate the water is released from ionic structure; therefore it is possible to measure the weight of the ionic compound and calculate its ratio to the liquid in the hydrate. The goal of the lab is to establish the identity
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Introduction: There are times when it is necessary to go through something both inside and outside‚ literally‚ to learn. This was exactly the case with virtual lab #11 where the experiment called for an examination of an earthworm’s exterior anatomy and interior anatomy. The earthworm as a whole is considered the dependent variable as we cannot change it. However‚ the labels to be placed can be moved so they are the independent variables. My hypothesis is quite elementary. I believe that after studying
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Introduction: To achieve genetic experiments with haploid organisms‚ genetic strains of different genotypes must be crossed from one another. Following fertilization and meiosis‚ the meiotic products can be analyzed as the ascomycete fungus‚ Sordaria fimicola. Sordaria can be used as a model to study meiotic segregation. The trait followed was the ascospore color. Ascospore color is a single gene trait therefore it is easily observed under a light microspore. Which allele is dominant is very tough
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