E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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“FACt.” Case: Vizio‚ Inc Frame: VIZIO is founded in 2002 by William Wang‚ with a startup capital of $600‚000. The company produces high-quality flat-panel televisions at affordable prices. From 2002 to 2007‚ it realizes continuous growth and expansion. VIZIOR earns razor-thin margins‚ at a time when other famous brands such as Sony and Samsung still focus on high-end customers and charge a very high price for flat-panel television. By the end of 2007‚ VIZIO reached $1.9 billion in revenue and
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Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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RUN‚ INC. Case 1) What are the practical differences in the accounting for a change in estimate and a correction of an error? Why might managements prefer one approach to another? What pictures do the two accounting presentations paint for readers outside the company? A change in estimate is a normal and ongoing process of a company. It usually arises from the appearance of new information that alters the current situation. Accounting for a change in estimate is treated prospectively. Companies
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A & E Bridal A & E Bridal (Bridal) provides product such as wedding gowns‚ bridesmaid dresses‚ shoes‚ jewelry‚ and other accessories. These goods are purchased from a few reliable outside distributors that specialize in the products that Bridal needs. Bridal first opened its doors in 1998 and now has fours stores located throughout the western United States. Each store employs 15 to 20 employees‚ about 70 in total‚ who hold positions such as manager‚ salesperson‚ seamstress‚ etc. There is a home
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aggregate wealth-creating capacity may be greater. [Because EVA‚ like ROCE‚ is also usually based on a "snapshot" or short time series of data.]•Is it valid to mix accounting and market measures as EVA does?Q3. Calculate EVA and MVA from Outsource Inc and whether it could be used as an incentive system for its employees. Ans: Operating Current Assets (OCA)= Current Assets - Short term investments= 438‚685 - 61‚047= $ 377‚638Operating Current Liabilities(OCL) = Current liabilities- Notes payable=
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Escherichia coli and Staphylococcus albus Some bacteria’s require a certain amount of light in order to function properly and there fore survive. Escherichia coli is found in the gut and intestines of mammals. Staphylococcus albus is generally a skin dwelling bacteria Aim: To test whether the amount and type of light that the bacteria’s E.coli and S.albus are subject to causes changes in their growth rates and habits. To test whether the growth rates and habits of the bacteria’s E. coli and S
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Abstract The purpose of this experiment was to study the transfer of genetic information on plasmid F’lac by using Escherichia coli. Plasmid transfer was measured by using two different methods. The first one was by using selection and contraselection with three antibiotics: streptomycin(which was replaced by naladixic acid for the second part of the experiment)‚ampicillin and kanamycin and the second one by using a colour indicator ( X-gal). As significant results‚ the percentage of transfer for
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Managing Outbreaks of an Infection M1: Explain how to manage an outbreak of infection in health or social care setting NICE and Department of Health Guidelines NICEandDepartment of Health guidelines set the standards for high quality healthcare. It encourages a healthy lifestyle for people. The NICE and Department of Health guidelines are also used by the NHS‚ Local Authorities‚ employers‚ voluntary groups and anyone else involved in delivering care and promoting wellbeing. The recommendations that
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virus can be. Epidemiological studies have reported a total of 44 countries which had Zika outbreaks from 2007 to 5 February 2016. This includes 33 countries that reported transmission in between 2015 and 2016. The most affected countries are Brazil with up to 1‚482‚701 cases reported since the outbreak began and Colombia with 20‚297 cases reported (up to 23 January 2016). Since the country’s first cases were detected in October 2015‚ there has been a rapid regional spread of the virus. By 4 February
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