Inhibition was not observed. Therefore‚ we hypothesized that this occurred because the binding ability of the AAoF compound is reduced compared to the unmodified AAF compound. We believe this to be due to the abrogation of the nucleophile-P1 amide hydrogen bond in the enzyme-substrate complex. Subsequently‚ we performed a broad range inhibition assay by increasing AAoF while keeping AAF constant. An expected trend was seen where the higher the AAoF concentration‚ the less activity
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Chapter 17: Alcohols and Phenols C O O H H sp3 alcohol pKa~ 16-18 phenol (aromatic alcohol) pKa~ 10 Alcohols contain an OH group connected to a saturated carbon (sp3) Phenols contain an OH group connected to a carbon of a benzene ring O H C C H C C O chemistry dominated by the keto form 76 enol keto H O H R O H R O R R O O R’ water alcohol R S H ether R S R peroxide R S S R’ thiols thioether disulfides
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2-methyl-2-butanol is most favorable for the reaction because when the leaving group leaves‚ it will leave a + charge on the carbon it was attached to‚ and a tertiary carbocation is the most stable. Another important requirement for a Sn1 reaction is a weak nucleophile attack group. Hydrochloric acid is a weak group because it has no negative charge‚ and works well for the reaction. Reaction for formation of 2-chloro-2-methylbutane The goal of this experiment was to form 2-chloro-2-methylbutane by addition
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This experiment involved a substitution reaction to perform synthesis of 2-phenylethanol to get (2-bromoethyl)benzene. Using NaBr in an acidic solvent of H2SO4 with H2O present‚ this synthesis was possible. Subsequent evaluations using TLC and then analyzed samples by Gas Chromatography were done to evaluate the results. Using SN2 reactions‚ primary alcohol is going to be converted to alkyl halide. Since the reaction can be reversed‚ the strong sulfuric acid was to make sure the product would indeed
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In the IUPAC system of nomenclature‚ functional groups are normally designated in one of two ways. The presence of the function may be indicated by a characteristic suffix and a location number. This is common for the carbon-carbon double and triple bonds which have the respective suffixes ene and yne. Halogens‚ on the other hand‚ do not have a suffix and are named as substituents‚ for example: (CH3)2C=CHCHClCH3 is 4-chloro-2-methyl-2-pentene. If you are uncertain about the IUPAC rules for nomenclature
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Tie-Dye Grignard Synthesis Abstract: 4-Bromo-N‚N-dimethylaniline underwent a Grignard reaction with diethyl carbonate to produce a type of the tie-dye chemical triarylmethane. This specific triarylmethane produces a vivid crystal violet color when dyed. The experiment was first heated under reflux to produce the necessary Grignard reagent as a grey liquid. It was then reacted with diethyl carbonate and hydrochloric acid to produce crystal violet. The resulting chemical was very absorbent to
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Chem 31.1 Post Lab 3 CARBONYL COMPOUNDS AND CARBOHYDRATES HYDROLYSIS OF POLYSACCHARIDES - - Susceptible to both nucleophiles and electrophiles Presence of C=O TEST FOR ALDEHYDES AND KETONES - - Reaction with 2‚4-DNP (Dinitrophenylhydrazine) Positive: Formation of yellow precipitate IODOFORM TEST - Carbonyl compounds: Methyl ketones will give a positive result Postive: Yellow precipitate (CHI3) MOLISCH’S TEST for carbohydrates - TOLLEN’S TEST – for aldehydes
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the alcohol to form an oxonium ion. The OH is converted to a better leaving group as the positive charge on the oxygen weakens the carbon-oxygen bond. The carbon-oxygen bond breaks during heating giving water an unstable carbocation. Without a nucleophile‚ the carbocation intermediate loses a proton from a carbon and forms a carbon-carbon double bond from the carbon-hydrogen electrons. The
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Non-classical ion. On water reactions: Why are some organic reactions accelerated at the water-organic interface?[2] What is the origin of the bond rotation barrier in ethane‚ steric hindrance or hyperconjugation? What is the origin of the alpha effect? Nucleophiles with an electronegative atom and one or more lone pairs adjacent to the nucleophilic center are particularly reactive. What is the nature of strong bonds between organic-sulfur (and higher chalcogen) compounds and gold?[3] Many mechanisms proposed
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substitution on aromatic compounds. 1. INTRODUCTION The most common reaction of aromatic compounds is electrophilic substitution. It is when an electron-poor reagent (an electrophile‚ E+) reacts with the electron-rich aromatic ring ( a nucleophile) and substitutes for one of the ring hydrogen. Electrophilic Substitution has several factors that affect its relative rates and this includes the following: substituents‚ solvent and temperature. Substituent like –OH and Br has
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