Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
Free PH Buffer solution Standard deviation
NaCl Effects on Peroxidase Activity My experiment was to see if adding NaCl to solution would have any effects on peroxidase activity. The materials that were used in this experiment were pH 7 buffer(DI water)‚ peroxidase‚ NaCl‚ guaiacol and hydrogen peroxide; added in that order. Blanks were created for each NaCl concentration‚ 0%‚ 5%‚ 7.5% and 10%. Each cuvette had .5ml of pH 7 buffer‚ 1ml of peroxidase‚ .02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the blank cuvettes‚
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Vanden Eynden CHM2510 AU13 Practice Midterm Exam #2 CHM2510 Practice Midterm Exam #2 This exam is designed to give you a small glimpse as to the format of the exams I write. The content of the exam has no direct correlation to the difficulty of the actual exam you will take. Use this exam as another problem set so you can get a little more practice in where multiple chapters are combined into one document. It would greatly benefit you to try doing this exam first WITHOUT ANY NOTES
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EXTRACTION OF INVERTASE FROM YEAST AND EFFECT OF pH ON ENZYMATIC ACTIVITY Jenelle C. Faustino‚ John Gambit B. Garcia‚ Fatima S. Jusay‚ Oliver Alexander B. Lao and Eunice L. Licudine Group 4 2 E Medical Technology Biochemistry Laboratory ABSTRACT Enzymes are substances that are produced by living organisms and act as catalysts in order to speed up or chance a chemical reaction without changing itself at the end of the reaction. Invertase was extracted first from baker’s yeast. Determination
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EXPERIMENT 3 Name: Flame Tests & Electron Configuration Pre-Laboratory Questions and Exercises Due before lab begins. Answer in the space provided. 1. Write electron configuration for the alkali metals Li‚ Na‚ K‚ and Rb. Li ____He 2s1_____________________________________________ Na ____Ne 3s1______________________________________________ K _______Ar 4s1___________________________________________ Rb _______Kr 5s1___________________________________________ 2. Write the electron configuration
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Complexometric Determination of Water Hardness 9/12/2013 Abstract: Two sets of acid-base neutralization titrations were conducted for experimental analysis. The first set of titrations was to standardize a solution manufactured in the lab. An approximate solution of Na2EDTA of 0.004 M was titrated against a known solution of 1.000 g CaCO3/L to deter mine to exact molarity of the Na2EDTA. Ca2+ + Na2EDTA → CaEDTA + 2Na+ The second set of titrations was to use the now standardized Na2EDTA
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Ashley Esparza Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a
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Did different conditions affect the Enzyme’s Efficiency? The hypothesis was “the more the imposed condition affects the mobility of the Enzyme‚ the fewer toothpicks will be broken”. This hypothesis proved true. This experiment manifested through multiple trials that the condition the enzyme had to endure definitely affected the Enzyme’s Efficiency. During this modeling experiment‚ it was observed that that the efficiency of the Enzyme over time created a line like that of a radical function
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