Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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was determined by red color of its flame. Unknown 1 was strontium chloride and unknown 2 was calcium chloride. The unknowns were found by comparing their flame colors to the known substances. Formula | Metal | Color Observed | Duration(sec) | CaCl2 | Calcium Chloride | Green | N/A | CuCl | Copper Chloride | Orange | N/A | FeCl3 | Iron Chloride | Sparkle | 6 sec | LiCl | Lithium Chloride | Pink | N/A | KCl | Potassium Chloride | Purple | N/A | NaCl | Sodium Chloride | Orange | 30 sec
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Chemistry Stage 3 Standards Guide Exemplification of Standards through the 2010 WACE Examination Chemistry: Stage 3 Standards Guide 1 2010/33606 Copyright © Curriculum Council‚ 2011 This document—apart from any third party copyright material contained in it—may be freely copied‚ or communicated on an intranet‚ for noncommercial purposes by educational institutions‚ provided that it is not changed in any way and that the Curriculum Council is acknowledged as the copyright owner. Copying
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Thirty test tubes One test tube rack Two test tube holders Two spatulas Three 250 mL beakers One stirring rod One medicine dropper Alcohol lamp or Busen burner Water bath One looped platinum or nichrome wire Label REAGENTS 0.5 M CaCl2 0.5 M BaCl2 M NaOH 0.1 M AgNO3 0.5 M KCl 0.5 M KBr 0.5 M KI 2 M NH4OH M KCN % H2O2 2 M H2SO4 MnO2 Saturated FeSO4 0.1 M NaNO3 CH3COOH 0.5 M Na2SO3 0.1 M KSCN 2 N KOH 0.5 M K4[Fe(CN)6] 0.5 M FeSO4 0.5 M FeCl3 0.5 M Al2(SO4)3
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also occur. Anhydrous CuSO4 is white‚ but upon exposure to air‚ the anhydrous salt absorbs water. This reaction produces blue CuSO4·5 H2O. In some cases‚ compounds can actually dissolve in their water of hydration. Salts such as calcium chloride (CaCl2)‚ calcium sulfate (CaSO4)‚ and magnesium sulfate (MgSO4)‚ can absorb so much water that they form solutions. These salts are said to be deliquescent. We often use anhydrous salts of this type as drying agents. Some hydrated salts such as Na2SO4·10
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Discussion Questions 1. Why do different metals have different characteristic flame test colours? The photons emitted during the test precisely match the quantum energy difference between the excited state and the ground state. For different elements the spacing between the ground state and the higher energy levels is different. So different elements have different flame test colours. 2. Most salts contain a metal and a non-metal. Look at the compounds we tested and determine whether it is
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1 a The diagram shows the parts of a hydrogen atom. [pic] Use words from the list to label the diagram. electron group nucleus symbol (2) b Hydrogen can be used as a clean fuel for cars. i When hydrogen burns in air‚ it reacts with another element. Complete the word equation for this reaction. hydrogen + ................... → water (1) ii Suggest one reason why hydrogen is called a clean fuel. (1)
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Introduction to Chemistry Laboratory: A Lesson on Tools‚ Techniques and Measurements PURPOSE: The purpose of this set of experiments (3 total) is to become familiar with the common types of laboratory glassware and equipment‚ and how to obtain and analyze data from these items. LEARNING OBJECTIVES: By the end of this experiment‚ the student should be able to demonstrate the following proficiencies: 1. Know which glassware (beakers‚ burettes‚ pipettes‚ graduated cylinders‚ flasks‚ etc) should
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Isolation: * 1 Cup Wheat Flour * Cheese Cloth * Iodine Solution * Water Qualitative Color Reaction Tests: * Isolated Gluten * 2.5 M‚ 3 M‚& 6 M NaOH * 10% & 20% NaOH * Conc. NaOH solution * 5% NaNO2 * 10% Na2CO3 * 2% Nitroprusside solution * 0.02% Naphthol solution * 1% Sulfanilic acid * 0.1% Ninhydrin solution * Conc. HNO3 * Conc. H2SO4 * Hopkins-Cole
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Gerona‚ Evelyn Chemical Reactions Experiment No. 7 Date Performed: Feb. 17‚ 2014 Date Submitted: Feb. 24‚ 2014 Mrs. Vitancol I. Theoretical Framework A chemical reaction is a process that leads to the transformation of one set of chemical substances to another. Classically‚ chemical reactions encompass changes that only involve the positions of electrons in the forming and breaking of chemical bonds between atoms‚ with no change to the nuclei (no change to the elements present)‚ and can often
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