commonly used techniques to test motility? C. Why are semi-solid media used to test for motility? D. Why might it be difficult to observe motility in a wet mount? E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube? F. For which of the organisms on the wet mount‚ if any‚ were you able to observe motility? G. For which of the organisms in the motility medium tubes‚ if any‚ were you able to observe motility? Questions A. Define the following
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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Commission‚ to produce legal documents related to the “isolated” outbreak‚ Although uncommon the FDA and prosecutors are working together to try to bring increased attention and punishment to companies found in outbreak violations.During the investigation E. Coli outbreaks raged through Chipotle stores across America. In total 57 consumers in twelve states were affected were hit with some strains of norovirus. Due to the investigations and outbreaks Chipotle
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Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
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BIO Microscopy‚ Mitosis‚ and Motility Lab Discussion Questions In the Microscopy‚ Mitosis‚ and Motility lab‚ the class used the Compound Microscope. The Compound Microscope helped examine the prokaryotic and eukaryotic cells‚ observe the different stages of mitosis‚ and compare modes of motility used by protists. The light compound microscope works with the aid of lenses such as the eyepiece. These lenses help keep the focus of the light on the eye. The fine and course adjustments also assist with
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The Figure 4.20 shows the profile growth of Escherichia coli type K-12 which was cultured in 20 g/L of glycerol treated with supplements from a carbon source under anaerobic condition and the medium supplemented with 10 g/L of trypton. About 10% working volume of inoculums were added to the medium for the profile growth process. The effects on growth curves of strains of Escherichia coli were investigated by measuring the concentration of succinic acid produced and its optical density by using HPLC
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The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
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Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths‚ pH‚ and in the Presence of an Antibiotic Dvora Szego‚ Elysia Preston Darcy Kmiotek‚ Brian Libby Department of Biology Rensselaer Polytechnic Institute Troy‚ NY 12180 Abstract The purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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Case #1 Business Ethics Case Studies: e-Health and Commercial Genetic Testing Veritas Diagnostics is a medical diagnostics company specializing in adult genetic susceptibility testing for a range of heritable and complex genetic conditions. Their main business involves testing for the mutations associated with cystic fibrosis‚ hereditary kidney disease‚ as well as breast‚ ovarian and colorectal cancer. They have a well-staffed‚ well-run‚ sophisticated genetics laboratory capable of
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