Breast Cancer Screening in Women with a Familial or Genetic Predisposition The role of MRI Borstkanker screening bij vrouwen met een familiaire of genetische predispositie De rol van MRI Cover painting: Heleen Vriesendorp Layout / cover design: Philip de Bruin Printed by: Optima Grafische Communicatie‚ Rotterdam ISBN: 90-8559-210-0 The studies described in this thesis were supported by grants from the Dutch Health Insurance Council (OG-9803) and Zon MW (6200.0005)‚ and were performed at the
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(Last accessed January 27‚ 2006). Meldrum‚ D. R. 2000. Automation for genomics: Part 1‚ preparation for sequencing. Genome Research 10(8): 1081– 1092‚ August. Meldrum‚ D. R. and L. E. Kavraki. 2004. Robotics and robotics-inspired algorithms for molecular and cellular biology: diagnostics‚ genomics‚ proteomics. NSF Workshop on Status of Robotics in the United States‚ Arlington‚ Virginia‚ July 21–22.
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Polymerase Chain Reaction (PCR)‚ discovered by Kary Mullis‚ has had an extraordinary impact on various aspects of biotechnology. PCR has revolutionized research and diagnostics-based molecular biology. PCR is a simple‚ accurate‚ and highly reproducible procedure. The technology introduced an important advantage to molecular biology. It provides the ability to start with a small amount of DNA and to be able to amplify it so that there will be a sufficient
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or molecule between the sides of the membrane 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Since the chemical formula for urea is (NH2)2CO and its molecular weight is about 60. It will not diffuse through the 20 MWCO membrane because it does not ionize. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your
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Biochemistry is a very interesting subject to study and eventually work in. From studying microorganisms to creating medicine to prevent alien viruses from infecting people‚ it has always been a fascinating subject to me. I became interested in this career because I was always interested in the science field. Ever since I was young‚ I always liked to experiment with different things. I found out about biochemistry by my chemistry tutor. In the first semester‚ I struggled with chemistry so I called
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We know the pressure will increase in the albumin beaker because it is more impermeable than glucose. The pressure in the beaker containing the albumin increased despite the increased concentration of glucose because albumin has a much greater molecular weight. Activity 4 1. Increasing the size of an opening allows more space for movement. For example‚ the more you open the nozzle of a hose the amount of water that comes out increases. 2. Powdered charcoal is too large to pass any of the
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Biochemistry‚ Ed 4. Brooks/Cole Cengage Learning * Snyder‚ L.‚ Champness‚ W.‚ 2007. Molecular genetics of bacteria‚ Ed 3. American society for microbiology * Varsani‚ A.‚ Heinemann‚ J.‚ Tisch‚ M.‚ 2013. BCHM231/202 S1 Lab Manual: Foundations in Molecular Biology. School of Biological Sciences. University of Canterbury. * Watson‚ J. D.‚ Baker‚ T.A.‚ Bell‚ S.P.‚ Gann‚ A.‚ Levine‚ M.‚ Losick‚ R.‚ 2008. Molecular Biology of the Gene‚ Ed 6. Cold Spring Harbor Laboratory Press‚ New York * Wei‚ H.‚
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Characterization of LIC13341 and its orthologs. The CDS LIC13341 from L. interrogans Copenhageni genome was cloned without its predicted signal peptide in pET28a expression vector and induced its expression in E. coli BL21. Induction of protein expression was evaluated using 0.5 mM IPTG where recombinant protein (43 kDa) overexpression was remarkably observed (Fig 2A). In order to further characterize LIC13341‚ polyclonal antibodies were generated in BALB/c mice against r-LIC13341. To check its
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Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
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OBSERVATION OF BACTERIAL CELLS VIA MICROSCOPE In this experiment‚ we have learned several basic molecular biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained
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