The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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result akin to populations of genetically facsimile indigent zigzag occurs in attribute instanter organisms such as bacteria‚ insects or plants echo asexually. Cloning in biotechnology refers to processes second-hand to open copies of DNA young (molecular cloning)‚ cells (cell cloning)‚ or organisms. The upon into the bargain refers to the extravaganza of parasynthetic copies of a prudence such as digital media or software. If you clone someone or some thing‚ it’s not going to be the exact same animal
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Chagas disease is a public health problem in Latin America with a great impact in Brazil. The latest estimates show that about seven million people in the world are infected by the kinetoplastid protozoa Trypanosoma cruzi (1). In spite of this‚ there are no vaccines against Chagas Disease (2). The current drugs used for treating this disease are effective only in the acute phase‚ with limited efficacy in the chronic stage of the disease and many toxic side effects (3) (4). Therefore‚ there is
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subject to intellectual property law (Wikipedia ‚the Free Encyclopedia‚2012) The term GM foods or GMOs (genetically-modified organisms) is most commonly used to refer to crop plants created for human or animal consumption using the latest molecular biology
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eHistory of biotechnology From Wikipedia‚ the free encyclopedia Biotechnology is the application of scientific and engineering principles to the processing of materials by biological agents to provide goods and services.[1] From its inception‚ biotechnology has maintained a close relationship with society. Although now most often associated with the development of remarkable drugs‚ historically biotechnology has been principally associated with food‚ addressing such issues as malnutrition and famine
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ACTIVITY ONE- Cell Transport Mechanisms and Permeability: Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. Molecular weight affects the rate of diffusion because the bigger the size of the molecule the longer it will take to diffuse. Membrane size is another variable that affects the rate of diffusion because if the membrane’s pores are small it will take molecules longer to diffuse though it than if the pores were larger. Also‚ membrane thickness
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Genetic Transformation of Bacteria Abstract The transformation of bacteria was successfully carried out using a plasmid carrying a gene that codes for green fluorescent protein‚ which gives a signature green glow reminiscent of a jellyfish. This gene‚ however‚ is only active when the sugar arabinose is present. A gene coding for antibiotic resistance was also found within the plasmid and served as a means to verify that transformation had indeed taken place. The hypothesis was that the bacteria
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could be re-joined in the laboratory. Researchers were quick to recognize that restriction enzymes provided them with a remarkable new tool for investigating gene organization‚ function and expression. As the use of restriction enzymes spread among molecular biologists in the late 1970’s‚ companies such as New England Biolabs began to search for more. Except for certain viruses‚ restriction enzymes were found only within prokaryotes. Many thousands of bacteria and archaea have now been screened for their
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DNA. Every now and then‚ technology has been used in every aspect in our life. But we know that technology has its advantages and disadvantages. Recently it’s now being bounded with our DNA. DNA technology according to the book is a collection of molecular biology techniques which aims at manipulating the DNA of an organism for a useful end. It involves genetic engineering‚ cloning‚ DNA testing and other techniques and process which uses the DNA‚ genes‚ chromosomes and other parts of our body. It has
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The explosion of molecular biology techniques that began in the mid-1970s (and continues today) has provided tools to examine the physical structure of DNA‚ its nucleotide sequence and how genes are read and regulated. One key tool is the ability to visualize DNA molecules and determine their length by using a technique called gel electrophoresis. Introduction to gel electrophoresis In gel electrophoresis‚ DNA fragments move through a porous matrix made of agarose‚ a gelatin-like substance
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