gave ions to support the conductivity for the gel to run. The uncut λ DNA would be added to labeled tube as a control DNA. By comparing the conformations of uncut DNA with the cut DNA‚ the completed digestion could be verified. Molecular marker‚ which was used for molecular weight calculation‚ was stored in another sample tube. The prepared sample contained puC18 DNA would be run as the degrade DNA control
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or Maxam/Gilberts sequencing. Introduction The capacity to segregate and amplify individual genes from an intricate genome using recombinant DNA technology technique like PCR has profoundly influenced ways scientists explored the obscurities in biology. Some of the major accomplishments of Recombinant DNA technology include the synthesis of medically essential proteins like insulin and developing new vaccines routes (Kingsman and Kingsman‚ 1988; Primrose et al.‚ 2001). Gene Cloning allows the
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify our foods to increase flavor‚ yield‚ nutritional value or shelf-life • Better understand human genetics • Clone cells or organs Molecular Biology’s Best Friends: Bacteria Why use bacteria? • They’re relatively simple organisms. • They reproduce very quickly and asexually (this means that the “daughter” cells will contain the exact same DNA as the “parent” cell). • It’s pretty easy
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the intron branchpoint activates the first step of pre-mRNA splicing. RNA Society. 516-528 2 3. F. He‚ C.-T. Wang‚ L.-T. Gou. (2009) RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos. Journal of Genetics and Molecular Research. 1466-1473 4 5. Dewang Zhou‚ Jin-Xiang Ren‚ Thomas M. Ryan‚ N. Patrick Higgins and Tim M. Townes. (2004) Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts. Nucleic Acids Research
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Animals to be classified: Hypocalymma Angustifolium‚ Hypocalymma Cordifolium‚ Xerochrysum Bracteatum‚ Xerochrysum Viscosum‚ Pteropus Poliocephulus‚ Petaurus Australis‚ Tachyglossus Aculeatus‚ Perameles Gunnii. Has roots‚ leaves and/or flowers? Yes (therefore Flora species) No (therefore Fauna species) Average height above 100 centimetres? Is the specimen nocturnal‚ its torso covered in fur? Yes No Yes Heart shaped leaves borne opposite each other and/or Newly grown stems
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biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The system used in gel electrophoresis consists of 3 major parts:
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band‚ spacing pattern of bands) you collected for the samples (fish‚ crab‚ food‚ protein ladder) in the appropriate columns. The approximate molecular weight of a band is determined by comparing the band of the food sample to the protein ladder bands. Sample Fish (1) Crab (2) Food (3) Protein Ladder (M) Number of Bands 5 2 2 6 Approximate Molecular Weight of the Bands Spacing Pattern of Bands 1 separated from 2 pair of bands spaced apart 2 bands at 45 and 29 kd 2 bands at 45 and
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Studying Life Characteristics of Living Things What are some characteristics of living things? No single characteristic is enough to describe a living thing. Some nonliving things share one or more traits with living things. Living things share the following characteristics: made up of units called cells reproduce based on a universal genetic code grow and develop obtain and use materials and energy respond to their environment maintain a stable internal environment change over time
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a molecular sieve is created such that the distance traveled in the gel toward the anode by any DNA fragment (all of which carry negatively charged phosphate groups in the presence of a basic buffer) is inversely proportional to its molecular weight. Further‚ such distance traveled has a linear relationship with the log of such fragment’s molecular weight. Since DNA consists solely of deoxyribonucleotides that differ only by their bases and each base pair has approximately the same molecular weight
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