Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes‚ namely‚ _lacZ_‚ _lacY_ and _lacA_‚ the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_‚ _lacY_ and _lacI_ would code for beta-galactosidase‚ galactosidase permease
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first movie ever to be encoded in the DNA of a living cell by Harvard researchers‚ where it can be retrieved at will and multiplied indefinitely as the host cells divide and grow. “What we’re trying to develop is a molecular recorder that can sit inside living cells and collect data over time.” said Seth Shipman‚ a neuroscientist at Harvard Medical School .Cellular events that are hard to observe in real time like the processes occuring during brain development can
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MATERIAL AND METHODS Recombinant protein production The recombinant T-Rp3 was expressed in E. coli BL21 (DE3) as previously described (Favaro et al.‚ 2014) and purified by a single step His-based affinity chromatography in an ÄKTA purifier FPLC (GE Healthcare). Protein was then dialyzed against 50 mM MES buffer (pH 6‚0) overnight at room temperature‚ frozen in liquid nitrogen and stored at -80 ˚C after 0.22 μm pore membrane filtration. Evaluation of DNA-protein interaction by gel retardation
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concentration values explain the bands thickness and color. The smear lastly is Gamma globulin although it has a close concentration as alpha 1 and beta globulin it has smeared zone and that’s due to its light molecular weight‚ so we conclude that another reason for the band thickness is the molecular weight of the
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able to detect and analyze proteins based on their ability to bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common technique used to separate proteins based on molecular weight under the influence of an applied electrical field and then used to prepare for the Western Blot (#1 Lehninger). The support medium used is a polyacrylamide gel and‚ it also used sodium
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Biology and Behavior Animal testing is not a problem in today’s society because it is beneficial to humans. It seems unethical to put animals through such pain and torture‚ but if we stopped it completely there would be a large amount of human lives lost. How could this be? The further advancements in medical and technological science is inevitable. Therefore‚ if the testing must be done to learn more about the brain and body‚ which species (animals or man) seems expendable for such testing. The
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connected with electromagnetic radiation. The most important will be the field of molecular imaging that is understood as the visualization‚ characterization and measurement of biological processes at the molecular and cellular levels in humans and other living systems. The techniques used for it include radiotracer imaging/nuclear medicine‚ magnetic resonance imaging (MRI)‚ optical imaging‚ ultrasound and others. Molecular imaging that is now used mostly in scientific laboratories will be probably
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Formal lab report: Abstarct: The Purpose of this experiment was to perform Agrobacterium-mediated transformation in Wild type Arabidopsis thaliana Columbia by using somatic plant transformation method. The whole process lasted for over a period of 11 weeks and we were successful in getting transformed plants. Agrobacterium tumefaciens strain containing pMP90 (Ti-helper) plasmid and pCAMBIA1391 (T-DNA) plasmid was used for this plant transformation. pCAMBIA1391 plasmid was constructed by cloning Brassica
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Results(Analysis Questions): Dyes: #4‚ #6‚ and #10 traveled the fastest because they contained a protein with a molecular weight that was smaller than the others. The dye mixtures contained a sample of DNA‚ RNA or other protein. These were negatively charged so that they traveled toward the cathode. There were twelve samples in all‚ labeled #1-#12. Agarose
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Catalog Number 166-0003EDU Week 7: pGLO Transformation Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes‚ and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation
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