1. Outline the process of DNA profiling (genetic fingerprinting)‚ including ways in which it can be used. 6 marks • sample of DNA obtained / leucocytes / from mouthwash / hair / other named source • satellite DNA / repetitive sequences used for profiling • amplification of DNA by polymerase chain reaction / PCR • cutting DNA into fragments using restriction enzymes • separation of fragments of DNA (by electrophoresis) • separation according to the length of the fragments • pattern of
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IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Sophie Harris Honors Biology Abstract #4 1/11/2016 Regenerative Biology Voss J. G.‚ Kump K. D.‚ Walker A. J.‚ Voss S. R. "Variation in Salamander Tail Regeneration Is Associated with Genetic Factors That Determine Tail Morphology." PLOS ONE. Ed. Henry H. Roehl. 3 July 2013. Available from http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067274 Scientists Gareth J. Voss‚ D. Kevin Kump‚ John A. Walker‚ S. Randal Voss conducted a study in order to determine tail regenerative growth
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Advanced Cell Biology II Step 1: How will you identify the “vital” cellular protein that the virus targets for degradation? (Hint: think proteomics). (3 pts.) First‚ since we know the viral RNA sequence and its targeting protein‚ we can investigate it in bioinformatics database‚ and can acquire some clues or hints about the target proteins. From the bioinformatics database‚ we might be able to find its structure‚ similarity with other proteins‚ functions‚ and binding domains. In other words
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The Ted Talk video entitled “Animations of Unseeable Biology” describes the process of DNA replication. As the speaker Drew Berry explains‚ this process is repeatedly occurring and is performed mechanically. He provides us an animation of how a chromosome is replicated and split with the help of microtubules. Microtubules are straight tubes that are made up of proteins and it resides within a cell. This hollow tube allows the mitosis phase to occur. The copying of DNA is transported across the microtubules
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MOLECULAR BIOLOGIST Education/Training Required: All molecular biologists must at least earn a bachelor’s degree in a related science field. Required courses usually include cell biology‚ evolutionary biology‚ genetics‚ ecology‚ general chemistry‚ biochemistry‚ organic chemistry‚ physics‚ molecular biology‚ multivariable calculus‚ and linear algebra. Most jobs for molecular biologists require a doctoral degree. During college‚ students may want to participate in science-related activities
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and synthetic biology are two techniques that go hand in hand but differ ever so slightly. It is this slight divergence that makes a world of a difference. Gene editing is defined as the process of editing an organism’s DNA by altering‚ removing or adding nucleotides to the genome. Whereas synthetic biology is defined as a combination of molecular biology and systems biology with engineering principles to design biological systems and bio-factories‚ or the use of molecular biology tools and techniques
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Biology Digestion IA 13/03/2013 Jason Gao Rationale: Digestion is the chemical breakdown of food into smaller components that are more easily absorbed. Digestion is a form of catabolism: a breakdown of large food molecules to smaller ones. When food enters into stomach‚ gastric juice starts protein digestion. Gastric juice mainly contains hydrochloric acid and pepsin. The pH value of hydrochloric in the stomach
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Introduction A sample of DNA found in a crime scene was provided along with five suspects. Their DNA was then processed using restriction enzymes and Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes
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NAME: ____________________________ DATE:____________________ Leaving Certificate Biology: Scientific Method Leaving Certificate Biology Scientific Method Please see Teachers’ Notes for explanations‚ additional activities‚ and tips and suggestions. Levels Students’ English-language skills should be developed to Level B1 during funded Language Support. Mainstream subject learning will require the development of skills at Level B2 if students are to cope with public examinations. Language focus
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