it will be able to grow in the ampicillin plates‚ but the non-transformed E.coli will not. Materials: Two microcentrifuge tubes 500 uL of ice cold 0.05 CaCl2 E. coli bacteria A sterile plastic loop A sterile P-20 micropipette 10 uL of pAMP solution A timer Ice A water bath 500 uL of Luria broth A spreading rod Four plates Incubator Procedure: Day before lab 1. Streak E. coli host cells for isolation. 2. Prepare six source plates. Day of lab 1. Get two microcentrifuge
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small iron scales that were tied together and bounded into armor plates with silk or leather cords. The helmet was made of eight to twelve iron plates‚ and below it was a five piece neck guard. The body of the armor had four parts. They did not have armor on their right arm so they could easily draw their bow. Knights wore armor made of chain mail. When bows and crossbows became more common they started to wear complete suits of plate armor made of metal. (Documents C‚ D) The Samurai followed the code
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The Earth’s structure The crust is the surface of the earth; it is a rock layer forming the upper part of the lithosphere. The lithosphere is split into tectonic plates. The plates move slowly on a layer called the asthenosphere. Meteorites can tell us what the earth is like-these are rock and metal fragments which fall to earth from space. We know the earth’s core is hot through hot spring geezers and volcano lava. 2 types of crust OCEANIC CRUST: it is found under the oceans; it’s thin and
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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difference between the theories of Plate tectonics and Continental drift. * Continental drift: The movement‚ formation‚ or re-formation of continents described by the theory of plate tectonics. * Plate tectonics: A theory that explains the global distribution of geological phenomena such as seismicity‚ volcanism‚ continental drift‚ and mountain building in terms of the formation‚ destruction‚ movement‚ and interaction of the earth’s lithospheric plates. Describe the 3 types of color patterns
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thoroughly in each of the concentration and air dried. Moist filter paper discs were placed in glass Petri plates (9 cm diameter) on which a single treated leaf disc was kept (for no-choice method). In choice method‚ in each Petri dish‚ along with a treated leaf disc‚ one untreated leaf disc of same size was kept. Single pre-starved (3-4 hours)‚ 7-day old larvae of S. litura was released into each Petri plate. Ten replicates were kept for each concentration. Leaf discs treated with solvent emulsified water
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incubation period‚ 1ml of the culture broth was appropriately diluted and plated on MSM-CV dye amended agar plates. The morphologically distinct bacterial isolates showing clear zones around their colonies due to decolorization of dye were selected for further studies. These isolates were screened for their ability to decolorize CV in MSM-CV dye amended agar plates. The screening procedure in agar plate was continued till the complete decolorization was obtained at different concentrations of CV dye. The
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Evolution of Australian Biota 1. Evidence for the rearrangement of crustal plates and continental drift indicates that Australia was once part of an ancient super continent Identify and describe evidence that supports the assertion that Australia was once part of a landmass called Gondwana including: Matching continental margins Gondwana once consisted of South America‚ Africa‚ Madagascar‚ India‚ Antarctica‚ Australia‚ New Zealand and New Guinea. Continental margin: zone between the ocean basin
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Earth’s internal layers… know physical concepts such as energy (kinetic‚ heat)‚ density‚ convection‚ bioaccumulation‚ biodegradation‚ photo-degradation‚ diffusion… Plate Tectonics – major Plate Tectonic principles; What are the 3 plate boundaries and their driving mechanisms? Where are trenches?... What are hot spots? What is our plate boundary here in SD? In which direction are we moving? Marine Provinces – the 2 continental margins and where they’re found… Seawater – the water molecule – characteristics
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QUEENSLAND ACADEMIES FOR SCIENCE‚ MATHEMATICS AND TECHNOLOGY International Baccalaureate Diploma Program Extended Essay in Biology Is Tea-tree Oil effective in controlling and minimising the growth of the Escherichia coli Bacteria? A study of the effectiveness of a Natural Antiseptic Candidate Name: Lois Adrienne Dianne Soriano Dulay Candidate Session Number: 003072-032 Word Count: 3997 Supervisor: Ms Kate Barry Date of Submission: 25th July 2011 Acknowledgments Firstly I would
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