Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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Introduction The science of microbiology is all about microorganisms‚ what they do and how they work. Microorganisms are ubiquitous‚ and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world‚ and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab
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SCOPE OF MICROBIOLOGY: What is Microbiology? | � Microbiology is the study of microorganisms� Microorganisms‚ roughly‚ are living things that are too small to be seen with the naked eye� Microorganisms cannot be distinguished phylogenetically from �macroorganisms� (see variety of microorganisms‚ below)� Microbiology is more a collection of techniques: aseptic technique‚ pure culture technique‚ microscopic observation of whole organisms‚ etc.� Microbiologists
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1. Title: The Process of Determining the Unknown Bacteria #9 Rachel Judecki July 5‚ 2011 2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol
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Introduction to Microbiology – Case Study 1 Kristen Alejos You are working as an emergency room nurse in Topeka when a mother brings in her 8 year old son because of severe‚ bloody diarrhea. When questioned‚ the boy said that it hurts when he goes to the bathroom and his mother said he had vomited in the previous two days. When asked about their previous movements‚ the mother and son both mentioned a backyard barbeque that had taken place 6 days earlier. The boy complained that his mother
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* The most important score is the Expect value‚ or E-value‚ which can be defined as the number of hits any random sequence (with the same length as yours) would have in the database. * E-values for good hits are usually written something like: 3e-42‚ which is the same as 3 x 10-42 ‚ a very small number * Bad hits are very common‚ and they have e-values in a more familiar form: for example‚ 0.004 or 1.2 * A really good e-values is less than 1e-180‚ which underflows the computer’s
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Service - Our Responsibility Water and Sewerage Authority (WASA) was established by an Act of Parliament in 1965 to manage the water and sewerage sector of Trinidad and Tobago. An essential component of this mandate is the delivery of a safe‚ reliable and efficient water supply to satisfy the demand of all sectors of the economy. Whilst over the ensuing years there has been a steady increase in demand‚ there has not been the commensurate investment in infrastructure and the identification of alternative
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State the principle of triple beam balance A beam balance measures mass as opposed to weight‚ so the mass you weigh will be the same on the moon as it is on earth. Gravity is taken out of the equation‚ unlike a spring balance that measures weight and would measure an article to be 1/6 of the weight on the moon as it would be on earth using the same spring balance that relied on gravity. The principle is that of moment‚ or turning force/torque)‚ calculated by force x distance. Fundamentally‚
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Ch. 1 Prokaryotes = bacteria ‚ archaea Eukaryotes = Fungi‚ protozoa ‚ algae (Growth:: Mitosis有絲分裂) Bacterial cell → Bacillus(rodlike) ‚ cocus (spherical)‚ spiral (curved) *P.S. 有時會star-shaped or square → 出現既形式: pairs‚ chains‚ clusters cell walls of bacteria → peptidoglycan (carbohydrate & protein complex) cell walls of plant and algal cell → cellulose Growth::Binary fission = bacteria reproduce by dividing into two equal cells bacteria 吸收nutrients from dead / living organisms/ photosynthesis
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NTRODUCTION Often in the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well
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