Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
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symbiotic relationship with its host‚ and for this reason it rarely causes diseases and is usually considered nonpathogenic (Avdic‚ Habes‚ and Avdic 3885). Recently though‚ the microorganism is becoming the common cause of nosocomial infections. In “Microbiology: with diseases by taxonomy”‚ Richard Bauman defines a nosocomial infection as “a disease acquired in a healthcare setting.” These infections are often found with implants and plastic items that have inserted into the body‚ such as catheters‚ pacemakers
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Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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Habituation of Exploratory Behavior in Rats Regarding Spatial Rearrangement of Familiar Objects in an Open Field Habituation of Exploratory Behavior in Rats Regarding Spatial Rearrangement of Familiar Objects in an Open Field Habituation is Although it might seem obvious that rats would habituate to an unfamiliar environment after repeated exposure‚ it is not as obvious how behavior would change as
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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