` Study Guide for Exam 1 Chapter 1: 1. What is a “microbe”? What groups of organisms are included in this category? Microbes (microorganisms) are minute living things that individually are too small to be seen with the unaided eye the group includes bacteria‚ fungi‚ protozoa‚ and microscopic algae‚ viruses Only minority of microorganisms are pathogenic(disease-producing) 2. What are some of the benefits provided by microbes? Decompose organic waste‚ producers in the ecosystem
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wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about
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LAB#: 20 SKILL: Planning and Designing OBSERVATIONS: A student is given a small beaker containing an unknown salt‚ x. The salt is crystalline‚ deliquescent and colorless. The student is asked to perform test and observation on the salt to determine the cation and anion present. HYPOTHESIS: Perhaps by using the flame test or reacting salt x with NaOH‚ or NH4OH the cation could be distinguished by observing the color changes or solubility while reacting salt x with H2SO4 or a mixture of copper
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
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CH4 Prokaryotic Cells No nucleus Plasma membrane Selective permeability lets molecules pass. Enzymes for ATP production. Photosynthetic pigment on folding (on chromatophores or thylakoids). Leakage occurs if damaged by alcohols‚ ammonium‚ and polymyxin antibiotics. Phospholipid bilayer‚ peripheral proteins‚ integral protein‚ transmembrane protein‚ sterols‚ glycocalyx Movement across Membranes Simple diffusion: Movement of solute from high concentration to low Facilitative diffusion: Solute
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Unknown 2 Scheme “ Fair game” ions Cations: Na+‚ K+‚ NH4+ ‚ Ca2+‚ Mg(OH2)62+ ‚ Al(OH2)63+ Anions: SO42-‚ HSO4-‚ NO3-‚ OH-‚ Cl-‚ CO32-‚ HCO3- Insoluble Compounds: Ca(OH)2‚ CaSO4•2H2O‚ CaCO3‚ MgCO3‚ Mg(OH)2 I. Describe Sample a. Quick description of sample. Ex: Phase‚ color‚ odor‚ crystalline‚ amorphous‚ gel-like‚ powdery‚ etc. II. Flame Test a. Part 1 i. Orange Flame: Na+ is present. K+ ‚ NH4+ ‚ Ca2+‚ Mg(OH2)62 ‚ Al(OH2)63+ may also be present. ii. Purple Flame: K+ is present (no Na+
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A medical microbiology lab performs testing on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory
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Review Questions 1. Which type of S. pneumoniae strain is the most virulent‚ encapsulated or unencapsulated? 2. What allows S. pneumoniae to adhere to cell walls‚ phosphorylcholine or polysaccharide? 3. Which infections are most commonly caused by S. pneumoniae‚ arthritis & peritonitis or meningitis & bacteremia? both 4. What can society do to help protect itself from antimicrobial resistance‚ increase or decrease antimicrobial usage? Decrease usage 1. Staphylococcus aureus‚
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The unknown soda ash from experiment 3 was used‚ to determine the weight for each trial we used the equation of (M of HCl) x (18 ml x 105.99) / (10 x 2 x Na2CO3 ). Which was equal to (0.01472 M) x ((18 mL X 105.99)(10 x 2 X 2.428 % )= 0.6 g. To start we had to rinse the beakers‚ electrode and the stirring bar with diluted water. The sample we needed was weighted to the closest 0.1 mg which we got was 0.3 for the first trial. The sample was transferred to a 250 mL beaker and dissolved in 70 mL of
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