Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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Classification for Functional Groups Alcohols Lucas test (Differentiates primary‚ secondary‚ and tertiary alcohols) Reagent: ZnCl2 in conc. HCl Observation: Rate of reaction (tertiary alcohol> secondary alcohol> primary alcohol) Procedure: 2mL Lucas Reagent in test tube+ 3-4 drops of alcohol‚ stopper‚ shake vigorously‚ NOTE time required (less than 10m mins only) to form an emulsion or separate layers. Oxidation (Confirms if alcohol is oxidizable: presence of H in C-OH bond) Reagent:
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Lab 2 Sara Rodriguez Period 2 Purpose: What is the more accurate way to measure volume‚ by using displacement or by using a ruler? Hypothesis: If the method of displacement is used ‚ then the results will be more accurate than measuring the volume using a ruler. Materials: -250 mL graduated cylinder -Triple beam balance -A weigh boat -Ruler -Samples Procedure: 1. Gather materials such as ‚ samples‚ weigh boat‚ triple beam balance ‚ graduated cylinder‚ ruler and water. 2. Take the materials
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African Diaspora and the World After reading the short story “Diaspora” by Joanne Hyppolite‚ I got a vivid understanding of how diaspora can have an effect on one’s identity. Over the past month we have studied many theories and concepts concerning race‚ gender‚ and politics. There are three theories that capture the essence of Joanne Hyppolite’s worldview as a Haitian growing up in America: intersectionality‚ identity‚ and diaspora. Individuals oftentimes experience the theory of Intersectionality
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Chemistry 121 Colligative Properties Lab Demonstration of Selected Calculations from Choice I Determination of Kf for Naphthalene To determine the Kf for naphthalene‚ we need to find the difference in the freezing point of pure naphthalene and the solution of 1‚4-dichlorobenzene in naphthalene. Let’s say that we did this experiment‚ used 1.00 g 1‚4-dichlorobenzene in 10.00 g naphthalene‚ and found that the freezing temperature of pure naphthalene was 78.2°C‚ while that of the solution was 75
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KASPERSKY LAB BUS 260A Marketing FALL 2012 Final Project Aghakarim Orujov‚ Alina Kurisheva‚ Natalya Amirova‚ Solongo Bayarma‚ Sergey Nikolenko Date of preparation: September 9‚ 2012 Date of submission: December 6‚ 2012 Executive summary Kaspersky Lab is an international group operating in almost two hundred countries and territories worldwide. The company’s headquarters are located in Moscow‚ Russia‚ from where it oversees global operations and business development. Kaspersky Lab offers
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Tuan Tai 1 Duong Tran Tuan Tai Professor Pham Quoc Loc ENGL - 1302 17 September 2014 Some nice quotes from unknown authors In my view‚ everyone love reading always notes their favorite quotes for their life’s guideline. To me‚ I am not really love reading but I love collecting the nice quotes. The quotes bring many meaningful‚ some are evident but some are vague. In addition‚ I also like to collect quotes from the Internet because I do not need to read whole a book and some of them never appear
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to cocoa bottom and an unknown girl notes to help on coursework. An unknown girl A traditional indian market scene‚ set in the evening‚ it was to hot to have markets in the day‚ lit up with neon lights She tries to capture the moment‚ she is getting involved in the culture by having her hand hennad. It is a darker shade of your own skin‚ suttle. She is watching this girl ‘icing my hand’(verb(icing)) ‘in the evening bazaar for a few rupees’ india money Unknown girl refers to the lady
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have a policy of Nondisclosure in an organization? a. It means that certain information can’t be made public under the company’s policy. 4. What Trends were tracked when it came to Malicious Code in 2009 by the Symantec Report researched during this lab? a. Swifi‚ Interrupdate‚ Fostrem‚ Kuaiput‚ Mibling‚ Pilleuz‚ Ergrun‚ Bredolab‚ Changeup‚ Induc 5. What is Phishing? Describe what a typical Phishing attack attempts to accomplish. a. Stealing online account information by posing as a legitimate company
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