Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology‚ 1822–1895) There
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04/29/2013 G-Unknown code Gram Positive-Micrococcus luteus Gram Negative- Klebsiella pneumoniae Unknown Paper The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth‚ Blood agar plate‚ MSA plate
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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Session #1 Lab 1 OBSERVING BACTERIA AND BLOOD D Haynes BIO-218A Microbiology Lab Friday‚ January 17‚ 5:00pm Objective(s): Our objective is to gain knowledge of the functions and operations of the microscope through observation of bacteria and blood slides that are available for this assignment. We are also learning to “see” and observe various bacteria and their shapes as well as live yogurt cultures‚ blood‚ plaque and cheek swab samples and their arrangements. We
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is lactose-negative. 15. C. Citrate test 16. the citrate test will differentiate E. coli from Klebsiella. Normally a battery of test would be performed to separate the numerous species in the family Eneterobacteriaceae. 17. B. The bacteria are citrate-negative. 18. the tube started off with the same green color‚ so no change occurred. This is in contrast to the tube on the bottom that was inoculated with a citrate-positive bacterium. this means that colony type 2 is normal E. coli
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True False 2. All cellular organisms can be placed into one of three __________‚ which include the Bacteria‚ Archaea‚ and the Eukarya. ________________________________________ 3. Archaea are cellular organisms that have unique cell membrane __________. ________________________________________ 4. Microbiologists study a variety of organisms‚ but all are considered either Bacteria or Archaea. True False 5. All eukaryotes have a membrane-delimited nucleus. True False 6.
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MICR 300A GENERAL MICROBIOLOGY 1 (4)‚ Fall 2014 Lecture (01) M/W 9:50 am – 11:05 pm‚ SH C244 Lab (02) M 11:15 am – 1:45 pm; Lab (03) W 11:15 am – 1:45 pm; ASCL 226 Instructor (Lecture): Dr. Moon H. Lee Office Hours: Mon/Wed 8:45 am-9:45 am; 11:15 am – 12:15 pm Office: BIOS 262‚ Tel: (323) 343-2064‚ Email: mlee@LABioMed.org Instructor (Lab): Dr. Hao Howard Xu Office Hours: Mon‚ 2:40 pm – 4 pm Office: Tel: (323) 343-2188‚ Email: hxu3@calstatela.edu
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Disposition/Food Safety: Overview of Food Microbiology July 8‚ 2011 Overview of Food Microbiology OBJECTIVES At the end of this module‚ you will be able to: 1. Explain the structural similarities and/or differences among Gram-positive and Gram-negative bacteria as well as their isolation and identification using serological‚ biochemical‚ and molecular techniques. 2. Identify the functions of the bacterial cell wall. 3. Identify the extrinsic and intrinsic parameters that affect bacterial growth
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between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic particle that infect the cells of a biological organism. Illustration courtesy of mc3cb.com. BACTERIA VIRUS STRUCTURE
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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