MBK – Lab Report Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They
Free Bacteria Staining Gram staining
Doyle and Schoeni (1984) reported 37 0C as the optimum temperature for growth of Escherichia coli 0157:H7 in Trypticase Soy Broth. This grew poorly in the temperature range of 44 to 45.5 °C which is generally used for recovery of E. coli from foods. Bettelheim (1998) designed Rainbow Agar O157 for the isolation and identification of EHEC. In this medium E. coli O157 was characterised by black colonies whereas O113 and some other EHEC strains were mauve‚ red or pink and indistinguishable from other
Premium Bacteria Microbiology Escherichia coli
1980 New Year’s Day From Mount Elgon in Kitum Cave‚ a virus first appears in Mr.Monet. Mr. Monet then goes to a hospital and exposes Dr.Musoke to the deadly disease. The virus is revealed to be Marburg by Dr.Silverstein‚ who sent samples to be examined at a research lab for special diseases. Marburg first appeared in an old city‚ in central Germany. Its first appearance was from a factory who shipped green monkeys to a different places that make vaccines from the kidney cells. The source of the
Premium Ebola Marburg virus Microbiology
The antifeedant activity of the extracts choice and no-choice test methods‚ after 24 and 48 hours in each test‚ against 7 day old (3rd instar) larvae of S. litura on castor leaves. Stock solutions of the test compounds were prepared in the carrier solvents. Further dilution was done in emulsified water by maintaining emulsifier (Tween – 80) level at 0.5 % to yield various concentrations. Castor leaf discs (4 cm diameter) were punched out from the washed and dried castor leaves. Leaf discs were dipped
Premium Bacteria Microbiology Escherichia coli
Do you think it is a good resource for the general public? Why or why not? The first page of the line above from the CDC is a bit confusing at first but the layout is relatively easy to manage (once you realize this is the right page). For the general public‚ a mission statement telling the reader the purpose of the resources given would be helpful. After selecting a section‚ the “second” page has a “better lay out”. The third click where the page layout with a side drop-down menu bar to the left
Premium Bacteria Antibiotic resistance Penicillin
Mechanisms of Silver Nanoparticle Action in Bacteria and Potential for Bacterial Resistance The antimicrobial activity of silver in general‚ and of silver nanoparticles in particular‚ is of significant interest because it appears to be independent of the strain of bacteria. Crucially‚ antibiotic resistant strains‚ MRSA (methicillin‚ or multiple-resistant Staphylococcus aureus)‚ E. coli O157‚ and others are affected by silver [6]. The reasons for this are not fully clear as yet‚ but could be related
Premium Bacteria DNA Escherichia coli
The purpose of this article is to express evidence that microcins mediate interspecies as well as intraspecies competition among Enterobacteriaceae in the inflamed gut. Enterobacteriaceae are known as a family of gram-negative bacteria that comprise of commensal organisms‚ primary and opportunistic pathogens that are a part of the leading causes of illness and death. Microcins are proteins that contain antimicrobial activity in vitro. The experiment in this article is not based on the collection
Premium Pie chart Escherichia coli Scientific method
The problem that was required to be investigated in this laboratory experiment was to determine which type of cleaning product would be able to prevent the most bacterial growth when placed in a neutral environment. In order to answer this problem‚ 8 different disinfectants were tested‚ including sterile water‚ and their after two days their zones of inhibition were measured and recorded. A hypothesis that was formulated before experimenting was‚ “If the type of disinfectant is Mouthwash‚ then it
Premium Bacteria Microbiology Water
Levishon and kleven (2000) discussed that the most dramatic disease of M. gallisepticum was chronic respiratory disease in meat type birds‚ often as one of several aetiological agents in a multi-factorial disease complex. Phenotypic variation of major surface antigens occurs at high frequency which was a probable explanation for chronic infection by M. gallisepticum despite a strong immune response. Bradbury et al. (2001) mentioned that avian Mycoplasma was caused by several pathogenic Mycoplasmas
Premium Bacteria Immune system Infectious disease
Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
Premium Oxygen Cellular respiration Bacteria