wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about
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MICROBIOLOGY What is the purpose of general growth media‚ selective media‚ and differential media? Give examples of each. General Purpose Media General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example
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References: 1. Beishir‚ L.: Microbiology in Practice: A Self-Instructional Laboratory Course‚ Fifth Edition. (Harper Collins: New York) 1991. 2. Jawetz‚ Melnick and Adelberg: Medical Microbiology‚ Nineteenth Edition. (Appleton and Lange: Norwalk‚ CT) 1991. 3. Tortora‚ Case and Funke: Microbiology: An Introduction‚ Fourth Edition. (Benjamin Cummings: Redwood City‚ CA) 1992. 4. Zubay: Biochemistry. (Addison Wesley:
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source of nutrients for microorganisms. With these characteristics of water‚ aquatic systems are more resistant to extreme environmental fluctuations. However‚ such water properties can be modified by the presence of dissolved substances. Aquatic environments are classified as ocean waters and inland waters (groundwater and surface water). Ocean waters contain approximately 35 ppt salt; freshwaters which include lakes‚ ponds‚ groundwater‚ river and spring‚ has 0.05 ppt salinity. Special habitats in
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fission = bacteria reproduce by dividing into two equal cells bacteria 吸收nutrients from dead / living organisms/ photosynthesis / inorganic substance 佢地可以swim by moving flagella Archaea if 佢地有cell wall → lack peptidoglycan Found in extreme environments -Growth:: Binary fission -cell wall → peptidoglycan 分3 main groups → methanogens + extreme halophiles + extreme thermophiles Methanogens → produce methane Halophiles → live in extreme env. eg. The Dead Sea Thermophiles → live in hot sulfurous
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Chapter 1: The Main Themes of Microbiology What is Microbiology? • micron = small and biologia = study of living things • Microbiology = study of small living things • How small is “small”? • What do we really mean by living? • Working definition: Study of entities too small to be seen with the unaided human eye. • (< 0.2 mm = 200 µm = 200‚000 nm) Types of Microbes Prokaryotes include Bacteria and Archaea Eukaryotes include Protista (Algae and Protozoa)‚ Fungi‚ and Animalia
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Importance of Microbiology| in Nursing Agriculture Pharmacy Advertisement Microbiology is a subject dealing with microbes and related concepts. Microbiology has come a long way since discovery of microbes and is presently of great help to mankind. It is used in health care‚ food production‚ diagnosis‚ production of alcohol‚ maintenance of sterility and cleanliness etc. Though the subject was initially limited to study of microbes and their characteristics or properties‚ latter it was explored to
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Microbes and Human Life Because of the presence of microbes in all walks of human life‚ there is constant interaction between microbes and humans. The vast majority of the bacteria in the body are rendered harmless by the protective effects of the immune system‚ and a few are beneficial. In fact‚ the relationship between microbes and humans is delicate and complex. Benefits of Microbes Microbes are useful for us in many ways in various industries. Production of Foods Microbes
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Lab Report #1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x‚ 40x‚ and 100x (oil immersion) objective lenses‚ which allowed for the identification of the different organism’s shapes and sizes. Purpose: The aim of this exercise is to equip the student with the knowledge
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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