Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
Premium Bacteria Microbiology Gram staining
The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
Premium Gram staining Staining Microbiology
are acidic dyes since they do not cross the cell wall/membrane particularly easily. A background stain is a stain used to stain everything other than the bacteria. The Gram stain is one of the oldest‚ most cost-efficient‚ yet most under-utilized‚ staining method used to identify bacteria. It consists of a basic dye (the primary stain crystal violet)‚ a mordant (Gram ’s iodine)‚ decolorizer (acetone:alcohol) and a counter stain (safranin). A mordant is a compound which helps hold the primary stain
Premium Bacteria Gram staining Staining
proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during
Premium Staining Gram staining
HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports.
Premium Gram staining Escherichia coli Bacteria
when one gains even a very basic knowledge of how diseases are acquired and spread. The purpose of this lab was to collect and observe microbes from environmental and human body samples by culturing them on the appropriate media using aseptic transfer techniques. Materials • Distilled water • Test tube • 6 Unopened packages of 1 sterile
Premium Bacteria Organism DNA
Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
Free Bacteria Staining Gram staining
the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate and confirm their identity. RESULTS Bacteriology – When grown on MacConkey agar‚ A had abundant growth of pink punctiform colonies that are circular and moist. Microbes A are gram-negative
Premium Microbiology Bacteria
Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
Premium Bacteria Escherichia coli
wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about
Free Bacteria Staining