Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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1 Student: ___________________________________________________________________________ 1. Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. True False 2. All cellular organisms can be placed into one of three __________‚ which include the Bacteria‚ Archaea‚ and the Eukarya. ________________________________________ 3. Archaea are cellular organisms that have unique cell membrane __________. ________________________________________
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Chapter 1. Microbiology – Diversity of Organisms Microorganisms- too small to be seen with the unaided eye “germ”- rapidly growing cell Microbes in our lives Pathogenic- disease causing Decompose organic waste Producers in ecosystem (photosynthesis) Produce industrial chemicals such as ethanol and acetone Produce fermented foods ( vinegar‚ cheese‚ bread) Produce products used in manufacturing (cellulose) and treatment (insulin) Designer Jeans: Made by Microbes? Stone washing- Tricoderma
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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Microbiology MCB 2010 Fall 2013 First Day of Classes: August 19 Last day to receive full refund: August 23 Last day to withdraw with grade of "W": October 24 Week 1 Topic Online Orientation Introduction to Microbiology (The Microbial World and You) The Unity of Living Systems (Anatomy of Eukaryotic and Prokaryotic Cells) Reading Assignments Chapter 1 Assignments to Submit Orientation Quiz Quiz 1 Quiz 2 Due Date Aug. 26 2 Chapter 4 Sept. 2 3 4 5 6 7 Chapters 5 and 6 Metabolism (Energy
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classification ● Genus is represented by staphylococcus ● Designing organisms in the industrial setting that can provide human products through genetic engineering or clean up environment waste through bioremediation is part of the sub discipline microbiology referred to as biotechnology ● A pathogen is the term used to refer to any disease causing microorganism ● The term ubiquitous is used to refer to the fact that microbes exists nearly everywhere on the earth ● A good hypothesis must be: able to be supported or rejected by
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References: Alonzo‚ C. 2008. A Laboratory Manual of Small-Scale Experiments for Independent Study of Microbiology. Englewood‚ CO‚ US: Hands-On Labs‚ Inc. OSMOSIS
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Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. Hypothesis By slowly narrowing down the bacteria by each test‚ I will be able to conclude the unknown bacteria by its special characteristics. MATERIALS AND METHODS An
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Name SOLUBILITY CURVES Answer the following questions based on the solubility curve below. Which salt is least soluble in water .. at 2O° C? 2. How many grams of potassium chloride can be dissolved in 200 g of water at 80° C? IO 3. At 40° C‚ how much potassium _ __nitrate coin be dissoiu$tl ^n 30D.g of water? ------W- ’1 80 70 ...- O --60 0 5© 40 4. Which salt shows the least change 30 In solubility from 0° - 100° C? 20 10 At 30° C‚ 90 g of sodium
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