Bacteria Morphology April 2‚ 2015 Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens
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Yun-Zheng Le‚2 and Alfred S. Lewin1 1 Department of Molecular Genetics and Microbiology‚ College of Medicine‚ University of Florida‚ Gainesville‚ Florida‚ United States Departments of Medicine‚ Endocrinology‚ and Cell Biology and Harold Hamm Diabetes Center‚ University of Oklahoma Health Sciences Center‚ Oklahoma City‚ Oklahoma‚ United States 2 Correspondence: Alfred S. Lewin‚ Department of Molecular Genetics and Microbiology‚ College of Medicine‚ University of Florida‚ Box 100266‚ Gainesville‚ FL
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Part I INTRODUCTION TO MICROBIOLOGY 1 The Evolution of Microorganisms and Microbiology CHAPTER OVERVIEW This chapter introduces the field of microbiology and discusses the importance of microorganisms not only as causative agents of disease‚ but also as important contributors to food production‚ antibiotic manufacture‚ vaccine development‚ and environmental management. It presents a brief history of the science of microbiology and an overview of the microbial world. The origin of life and
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consists of pili (Todar‚ n.d). The pili on this pathogen work with the toxin‚ adenylate cyclase‚ to bind Bordetella pertussis to human respiratory epithelium (Hartsock‚ n.d.) While this pathogen does produce toxins‚ it does not form endospores (Finger & von Koenig‚ 1996). Endospores allow cells to survive in very complex environments‚ and they are dormant cells. This means that Bordetella pertussis isn’t capable of surviving in harsh environments. Spores are
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EXPERIMENT 1: PREPARATION OF BIOLOGICAL MATERIALS FOR MICROSCOPIC EXAMINATION Introduction: Cell is the smallest structural and functional unit of an organism‚ and is often called the building blocks of life. All organisms are composed of one or more cells‚ they are classified into 2 groups which are unicellular organisms and multicellular organisms. Plants and animals are made up of cells too. There are two basic classes of cells‚ prokaryotic and eukaryotic cells. The structurally simpler‚ prokaryotic
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STERILIZATION AND DISINFECTION Definitions Bacteriostatic Agent: An agent that inhibits the growth of bacteria‚ but does not necessarily kill them. Bactericide: An agent that kills bacteria. Most do not kill Endospores. Sporicide: An agent that kills spores. Definition STERILIZATION The process of freeing an article from microorganisms including their spores. By definition‚ it effects a reduction in the number of microorganisms (more than 99.9999% are killed). Methods
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giant cluster tightly packed together. 4. What is the difference between direct and indirect staining? (1) Direct staining stained bacteria with basic dye (methylene blue‚ carbol fuchsin‚ crystal violet‚ etc) which its coloring power is in positive ion‚ and reacts with the negatively charged cell. Thus‚ the bacterial cell will be stained with the basic dye’s color. In other words‚ direct staining
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Takahashi K (2002). Lignin–degradation ability of litter decomposing basidomycetes from picea forest of Hokkaida Myco.sci. (41): 105 – 110. 3. Domsch KH‚ Gaws W‚ Anderson TH (1980). Compendium of soil fungi 4 5. Pelczar MJ‚ Chan ECS‚ krieg NR (1993). Microbiology: Concept and Application International edition McGraw-Hill‚ USA 6. Wall DH‚ Virginia RA (1999). Controls on soil biodiversity insights from extreme environments. Appl. Soil Ecol. (13): 137–150. 7. Fawole and Oso‚ 2001 Results and tables
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tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon
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Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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