* Microbiology: Study of microorganisms Most microorganisms are pathogenicFalse The majority of bacteria are harmless True Total number of bacterial species: about 10 million to a billion What is a microorganism? An organism or virus too small to be seen without a microscope. (Smaller than 0.5 mm) Ex: Bacteria‚ Fungi‚ Protozoa‚ Algae‚ and Viruses Microbiom: collection of microbes Human cells vs. Microbial cells = 1:10 Which statement about Michaelangelo is not correct? A. He was
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Differential Staining Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each‚ learn and employ the streak and pour dish techniques‚ and generate a pure culture of a specific organism. Set Up: For this experiment I needed: 1 Distilled water‚ 1 Paper towels‚ 1 10%-bleach or 70% alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable
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Ch. 1 Prokaryotes = bacteria ‚ archaea Eukaryotes = Fungi‚ protozoa ‚ algae (Growth:: Mitosis有絲分裂) Bacterial cell → Bacillus(rodlike) ‚ cocus (spherical)‚ spiral (curved) *P.S. 有時會star-shaped or square → 出現既形式: pairs‚ chains‚ clusters cell walls of bacteria → peptidoglycan (carbohydrate & protein complex) cell walls of plant and algal cell → cellulose Growth::Binary fission = bacteria reproduce by dividing into two equal cells bacteria 吸收nutrients from dead / living organisms/ photosynthesis
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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Microbiology MCB 2010 Fall 2013 First Day of Classes: August 19 Last day to receive full refund: August 23 Last day to withdraw with grade of "W": October 24 Week 1 Topic Online Orientation Introduction to Microbiology (The Microbial World and You) The Unity of Living Systems (Anatomy of Eukaryotic and Prokaryotic Cells) Reading Assignments Chapter 1 Assignments to Submit Orientation Quiz Quiz 1 Quiz 2 Due Date Aug. 26 2 Chapter 4 Sept. 2 3 4 5 6 7 Chapters 5 and 6 Metabolism (Energy
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Chapter 1: The Main Themes of Microbiology What is Microbiology? • micron = small and biologia = study of living things • Microbiology = study of small living things • How small is “small”? • What do we really mean by living? • Working definition: Study of entities too small to be seen with the unaided human eye. • (< 0.2 mm = 200 µm = 200‚000 nm) Types of Microbes Prokaryotes include Bacteria and Archaea Eukaryotes include Protista (Algae and Protozoa)‚ Fungi‚ and Animalia
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References: Alonzo‚ C. 2008. A Laboratory Manual of Small-Scale Experiments for Independent Study of Microbiology. Englewood‚ CO‚ US: Hands-On Labs‚ Inc. OSMOSIS
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Terms to Know Decomposer - Breakdown of dead matter and wastes into simple compounds Prokaryote – No nucleus - microscopic‚ unicellular organisms‚ lack nuclei and membrane-bound organelles Pathogen - Microorganisms that do harm Eukaryote - unicellular (microscopic) and multicellular‚ nucleus and membrane-bound organelles alcohol - archaeobacteria - Prokaryotic single-celled organisms of primitive origin that have unusual anatomy‚ physiology‚ and genetics‚ and live in harsh habitats;
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virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution * Applied biological science * What microorganisms do; their role in the nature? * Causative agents
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