GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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fixation Gas vacuoles: protein covered cylinder Magnetosomes: Iron oxide kills H2O2 Nuclear Area: no nucleus has nucleoid Ribosomes make protein Endospores: resting‚ not growing‚ resistant to desiccation‚ heat‚ chemicals. Bacillus‚ Clostridium a. Sporulation: Endospore formation b. Germination: return to vegetative state c. After endospore forms bacterial cells die d. Dormancy: Sporulation occurs surrounding cell dies away. Temp. gets better it germinates and continues its growth Eukaryotic
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Theories of Staining Techniques Staining bacteria with different dyes via staining techniques‚ allows in distinguishing the microorganism from its backgrounds. Also‚ helps in studying different internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties
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Title: Staining Introduction: Microorganisms are small and colorless‚ invisible to unaided eyes. When observing them under microscopes‚ we use various methods to make microbes apparent. One of the most important methods is staining. Staining techniques play an essential role in the studying of microorganisms; they help to reveal characteristics of microbes‚ such as their morphologies‚ sizes‚ arrangements‚ chemical components and many more. The purposes of the experiments are to be
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Microorganisms are classified according to their structure. By means of flow charts‚ diagrams and tables explain the differences between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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cell wall. The dye will be locked in the spore wall‚ which has peptidoglycan deeper in the walls. The keratin forming the outer portion of the endospore wall resists dye. The heating of the bacteria will make the spore wall more permeable to the malachite green‚ and it then attaches to the peptidoglycan. Thus‚ we can detect the bacteria that have the endospore based on this test. On a clean glass slide a drop of distilled water will be added to the slide. A smear will be prepared on this droplet by
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Disposition/Food Safety: Overview of Food Microbiology July 8‚ 2011 Overview of Food Microbiology OBJECTIVES At the end of this module‚ you will be able to: 1. Explain the structural similarities and/or differences among Gram-positive and Gram-negative bacteria as well as their isolation and identification using serological‚ biochemical‚ and molecular techniques. 2. Identify the functions of the bacterial cell wall. 3. Identify the extrinsic and intrinsic parameters that affect bacterial growth
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Contributors to Microbiology Pioneers of Microbiology I. Antonie van Leeuwenhoek (Oct. 24‚ 1632- Aug. 30‚ 1723) A. In 1676 Leeuwenhoek saw tiny organisms in water‚ he was the first man to observe and describe bacteria accurately. He discovered microscopic nematodes‚ blood cells and sperm. He also made over 500 microscopes to view specific objects. B. Microbiology is concerned with the study of all forms of life that are too small to be seen with the naked eye. Antonie’s work
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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