Session #1 Lab 1 OBSERVING BACTERIA AND BLOOD D Haynes BIO-218A Microbiology Lab Friday‚ January 17‚ 5:00pm Objective(s): Our objective is to gain knowledge of the functions and operations of the microscope through observation of bacteria and blood slides that are available for this assignment. We are also learning to “see” and observe various bacteria and their shapes as well as live yogurt cultures‚ blood‚ plaque and cheek swab samples and their arrangements. We
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BIOL 1020 Lab Assignment: Virtual Microscopy Start by re-saving this file as follows: lab_surname_virtualmicroscopy.rtf‚ substituting your own surname. Type your responses to the questions below where indicated. Remember to save your work frequently. Viewing Magnification‚ Field of View‚ and Specimen Size 1. On the UD Virtual Compound Microscope‚ examine the onion root tip slide at 400X viewing magnification. Ensure that the image is centred. What is the approximate size of an onion root
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MICROBIOLOGY What is the purpose of general growth media‚ selective media‚ and differential media? Give examples of each. General Purpose Media General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example
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Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor
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CH4 Prokaryotic Cells No nucleus Plasma membrane Selective permeability lets molecules pass. Enzymes for ATP production. Photosynthetic pigment on folding (on chromatophores or thylakoids). Leakage occurs if damaged by alcohols‚ ammonium‚ and polymyxin antibiotics. Phospholipid bilayer‚ peripheral proteins‚ integral protein‚ transmembrane protein‚ sterols‚ glycocalyx Movement across Membranes Simple diffusion: Movement of solute from high concentration to low Facilitative diffusion: Solute
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UNKNOWN LAB REPORT UNKNOWN NUMBER 54 Ron Williams 7-28-05 Bio 205 Prof. Curlee Fall 2005 Purpose There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory
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Review Questions 1. Which type of S. pneumoniae strain is the most virulent‚ encapsulated or unencapsulated? 2. What allows S. pneumoniae to adhere to cell walls‚ phosphorylcholine or polysaccharide? 3. Which infections are most commonly caused by S. pneumoniae‚ arthritis & peritonitis or meningitis & bacteremia? both 4. What can society do to help protect itself from antimicrobial resistance‚ increase or decrease antimicrobial usage? Decrease usage 1. Staphylococcus aureus‚
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learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation and recording of data the unknown organism can be found. Materials and Methods The procedure for all tests performed was taken from Leboffe and Pierce’s Microbiology: Laboratory Theory
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7351_2006_1_S03 Rajiv Gandhi University of Health Sciences‚ Karnataka PM.D. [MICROBIOLOGY] DEGREE EXAMINATION Time: 3 Hours [Max. Marks : 100] PAPER I – GENERAL MICROBIOLOGY AND IMMUNOLOGY QP Code: 7351 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. Answer all questions LONG ESSAYS 1. 2. 2 X 20 = 40 Marks Discuss the production‚ properties and uses of monoclonal antibodies Discuss various in vitro antibiotic sensitivity
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virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution * Applied biological science * What microorganisms do; their role in the nature? * Causative agents
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