Lab #1 (16th October‚ 2012) Safety in the Microbiology Lab * Clinical specimens received from patients pose a hazard to personnel health because of the infectious agents they may contain. * Spurred by the spread of HIV/AIDS‚ the CDC has recommended safety precautions concerning the handling of patient materials by health care workers. * Universal precaution (or blood and body fluid precaution). * Emphasis on good knowledge and common sense. * Safety is everybody’s business
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Lab #1 ‐ Introduction to the Microscopy & Observation of Prokaryotic and Eukaryotic Cells Introduction Many of the cells and organisms that you will be studying are at the lower limits of visibility of light microscopes; therefore‚ it is extremely important that you attain critical lighting and focussing. It is also important to handle the microscope competently to avoid damaging either the microscope or the preparation you are studying
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1. Introduction Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques ‚ according to the specimen used and the scope of the experiment. Two specimens‚ stained and unstained‚ containing CHO cells‚ were prepared‚ examined and analyzed under the microscope using bright field (HF)‚ dark field (DF) and phase contrast (PH) settings. In addition‚ the four phases of cell division cycle were estimated.. Bright
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By examining blood smear by microscopy it can detect infections whether they are viral or bacterial. Blood smears are examined by looking at the white and red blood cells appearances. The aim of the experiment is to compare a normal blood smear and its pathological features to those with an infection or abnormality by the use of microscopy looking at the cells involved with blood. A normal blood smear would contain 3 to 4 white blood cells per field in microscopy. If there is an increase in the
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Microscopy Cell are very Small Metabolic requirements set upper limits on the size of cells The surface area to volume ratio of a cell is critical As the surface area increases by a factor of n2‚ the volume increases by a factor of n3 Small cells have a greater surface area relative to volume Figure 6.7 Surface area increases while total volume remains constant 5 1 1 Total surface area [sum of the surface areas (height width) of all box sides number of boxes] 6 150 750 Total
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Please answer the following questions. Your answer for each question should be a minimum of 150 words (half page). Please number your answers with the number of the question you are answering. 1. Koch was the first scientist to prove that bacteria actually cause disease. He scientifically demonstrated that a disease is caused by a particular organism. He created four general guidelines to aid in identification of disease causing pathogens. These guidelines developed from his work with purified
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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Virtual Lab 1: Virtual Microscopy A. Estimate the size (length and width) of these microscopic objects in micrometers (microns): 1. An E. Coli cell. 3x 0.6 μm = 1.8 μm 2 A mitochondrion. 4x 0.8 μm = 3.2 μm 3. A Red blood cell. 8 μm 4. A virus. 220 nm = 0.00022 μm 5. A water molecule. 275 pm = 0.000275 μm B. 1 Describe three differences between prokaryotic and eukaryotic cells. The three differences between prokaryotic and eukaryotic cells are: Eukaryotic cells contain a nucleus inside
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Kevina Smith Lab 1: Microscopy and the Metric System Part A: Microscopy Purpose The purpose of this experiment was to learn how to use a microscope correctly and perform wet mount slides accurately‚ thus becoming more familiar with the microscope. Hypothesis It would be hard to use the microscope without any kind of previous training and the parts of the microscope and their functions must be learned in order to use it properly. Materials & Methods Materials: 1. Filter
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