The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Chemicals Hordenine and methyl jasmonate were purchased from Tokyo Chemical Industry (Tokyo‚ Japan). Dimethyl sulfoxide (DMSO) was obtained from Sigma-Aldrich (Tokyo‚ Japan). Plant materials Two-year-old seedlings of Vitis vinifera cv. Koshu were cultivated in perlite:vermiculite (1:1) soil in a growth chamber (11.8 Wm-2 for 14 h in a day) at 27 °C. Seedlings of Fragaria×ananassa cv. Nyoho were grown in 55% peat moss‚ 10% perlite‚ 5% vermiculite‚ and 30% decomposed granite soil at 25 ºC in a greenhouse
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Among one of the cities in Orange County and with a population of 625‚000‚ and located nearly 32 miles southeast of Los Angeles‚ the City of Orange government is formed under a council-manager; whereas every two years a mayor is elected and four city council members are nominated to a four-year term interchanging every two-years. Additionally‚ the city offers support to its citizens in the form of economic development programs and marketable and residential rehabilitation via agreements with the
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THE PROBLEM This study aims to extract antioxidants from orange peels to use as natural preservatives especially for mangoes. Specifically‚ this study attempted to answer the following question: 1. How could the extracted antioxidant help the mango preserve its freshness? 2. Can the orange peels really be use as a alternative preservative for the mango? RESEARCH HYPOTHESIS Ha: The extracted preservative from the orange peel has no effect on the mango. Ho: The
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Electrolyte Challenge Sport Drinks vs. Orange Juice By: Clem L. Vinegar Jr. Mr. Wells February 2‚ 2013 I. Project Question- The question I am trying to answer is which drink between water‚ Gatorade‚ propel‚ PowerAde‚ and a homemade drink. II. Hypothesis- My hypothesis is that orange juice will have more electrolytes than the other drinks because of the natural electrolytes it contains from oranges. I also think that Gatorade will be the most preferred drink
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A Clockwork Orange Authors who write of other times and places help us to better understand our own lives. Discuss A Clockwork Orange in terms of that statement. A "clockwork orange" can be described as something that has a convincing outer appearance yet in the inside is merely controlled by outer influences‚ such as a clock set in motion by its owner. In A Clockwork Orange‚ Anthony Burgess takes us into the future where violent criminals are forced to be "good‚" and introduces us to
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This report focuses on the problem of the current state of the California University State University‚ Northridge (CSUN) Orange Grove pond. It will provide an informational analysis on what sort of organisms thrive in a pond‚ how they are able to thrive‚ and how to manage a pond. It also includes strategic ways to maintaining a pond‚ such as using a efficient water filtration system‚ introducing eco-friendly biolife to control algae population. There will be a recommendation section that will discuss
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The girl with the orange bow‚ built up her courage and moved her cart towards the bag boy’s checkout line. “Hey there!” The girl in the orange bow grew nervous and her heart was pounding faster than it should be. It was pounding like if she just finished running a mile. “Hello miss. How may I assist you?” The bag boy cleared up his throat and smiled at the girl. He adored her. Everything about her he loved. Especially her eyes and her bow almost spoke out her personality. He couldn’t keep his
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Ghassan Kanafani in: “ The Land of the Sad Orange” Ghassan Kanafani was born in Akka Palestine in 1936 and died‚ as a result of an Israeli bomb planted to his car on 8th July 1972. His Danish wife Annie‚ described the event saying: “…We used to go shopping together every Saturday morning‚ on that day he accompanied his niece Lamees. A few minutes after they left‚ I heard the sound of a huge explosion. I ran but only saw remanence of our exploded small car. Lamees was a few meters away from the spot
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Daniel Ahn The Genius of Walt Whitman One of America’s greatest poets in history‚ Walt Whitman‚ wrote the masterpiece “When Lilacs Last in the Dooryard Bloom’d” which is a literary masterpiece that showcases sincere and genuine feeling through his facility of words. Whitman utilizes certain words to enhance his diction to create a somber yet hopeful tone and parallelism to create a more melodious elegy that has lasted through the centuries. Whitman uses his diction to create a dark melancholy
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