colonies due to their broad staining spectrum. (Black‚ 2008‚ pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye‚ the crystal violet‚ whereas the gram negative bacteria loses the primary dye once its washed with a decolorizing solution but takes on the color of the counterstaining dye of
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Reactions of Carbonyls-Aldehyde/Ketone Analysis: Introduction: The carbonyl group is a rich source of many important reactions in organic chemistry‚ with two fundamental properties that are primarily responsible for its diverse chemistry. The first is the polarization of the. Carbon-oxygen pi bond‚ owing to the relatively high electro negativity. The second property of a carbonyl function is to increase the acidity of the alpha-hydrogen atoms‚ which are the hydrogen’s
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of the amount of light to the absorbance is called spectrophotometer. Spectrophotometer used to test the sample by passing through the light it’s worked to measure the light that passing through. Biological substances such bromophenol blue and methyl orange‚ are the common substances to be used in testing of interaction of substance with the light. These solutions called pigments where they usually can absorb the visible of light that passing through it. The cleared compounds or solution also can
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candies are different colors? Many candies contain colored dyes. Bags of M&Ms or Skittles contain candies of various colors. The labels tell us the names of the dyes used in the candies. But which dyes are used in which candies? We can answer this by dissolving the dyes out of the candies and separating them using a method called chromatography. Research Candy Chromatography is the method of analysis of separating and comparing dyes used in candies and food coloring. It is also a proven method
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EXPERIMENT 2: SYNTHESIS OF ORANGE II (2-NAFTHOL ORANGE OBJECTIVE 1. To produce dye based to the coupling of diazonium. 2. To understand the characteristic of dye orange II. INTRODUCTION The first dye was introduced by William Henry Perkin on 1856. It can be produced by the coupling of diazonium sulfanilic acid with nafthol in alkaline solution.. This was the common method used to produce dye nowadays. In this experiment‚ dye produced was the orange II. Azo compound is compound that contain
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Experiment # 4: Analysis of Soda Ash Experiment # 5: Volumetric Analysis of a Carbonate – Bicarbonate Mixture Submitted by: Eugenio December 2012 Department of Chemical Engineering University of Santo Tomas España‚ Manila Abstract Soda ash is the common name for sodium carbonate (NaCO3)‚ a chemical salt derived from carbonic acid. It is frequently used in manufacturing‚ industry‚ and in domestic chores. Glass production is one of the primary industrial uses for sodium carbonate. It is also
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Beaker‚ 250 cm3 Retort stand Burette clamp Burette‚ 50cm3 Pipette‚ 25cm3 Pipette filler Beaker‚ 100cm3 White tile Conical flask‚ 250cm3 Reagents used: Distilled Water Hydrated Sodium Borate 1.0 M Hydrochloric acid Screened methyl orange Sodium Borate solution Method: Prepare a standard solution of hydrated sodium borate (250cm3 solution) Place the weighing bottle on the balance and record the mass of the empty bottle. Add approximately 4.80 grams of the hydrated sodium borate
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following are chemical tests that we will use in this class. Some cover two related functional groups (such as the bromine test for alkenes and alkynes)‚ while other only a specific subcategory of that functional group (such as the iodoform test for methyl ketones). In some cases there can be false positives (compounds which do not have the functional group still give a result test) or false negatives (compounds which have the functional group give a negative result). If your results do not match
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water methyl orange hydrochloric acid Apparatus: measuring cylinder graduated flask pipette beaker conical flask burette glass rod filter funnel Procedure: 1) 40.00 of the solution of potassium hydroxide is placed into a 250 graduated flask make up to the mark with deionized water . The mixing solution is shade well to obtain a homogenous solution. 2) 25.0 of the diluted potassium hydroxide solution is pipetted into a conical flask . 1-2 drops of methyl orange is added
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be the diluted window cleaner solution. Methyl orange will be used as the pH indicator of this reaction. Apparatus Burette‚ 50 cm3 Small beaker / Weighing bottle Burette stand Wash bottle Conical flask‚ 100 cm3 Dropper Volumetric flask‚ 250 cm3 with stopper Funnel Volumetric pipette‚ 25 cm3 Standardized sulfuric acid‚ H2SO4(aq)‚ ~ 0.01 M Pipette filler Window cleaner solution White tile Methyl orange Procedures Part A Preparation of
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