Tie-Dye Grignard Synthesis Abstract: 4-Bromo-N‚N-dimethylaniline underwent a Grignard reaction with diethyl carbonate to produce a type of the tie-dye chemical triarylmethane. This specific triarylmethane produces a vivid crystal violet color when dyed. The experiment was first heated under reflux to produce the necessary Grignard reagent as a grey liquid. It was then reacted with diethyl carbonate and hydrochloric acid to produce crystal violet. The resulting chemical was very absorbent to
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Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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to a blue green solution if it reacted positively. This was seen in all the sample used in this test except for acetone. Whereas Dinitrophenylhydrazone (2‚4-DNP) Test was preformed to test for aldehydes and ketones which would result to a yellow orange precipitate if it was positively reacted. All the compounds subjected to this test namely
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Introduction Spectrophotometer is an optical instrument that measures and shows how much light energy is transmitted by a substance in solution at different wavelengths of radiant energy. Light passes into a monochromator where only a very narrow range of wavelengths can pass through. From there‚ light is transmitted through a sample solution‚ and on to a phototube where the light energy is converted to an electric current that is registered on a meter. To measure the amount of light absorbed
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Name : Joanne Wong Student ID : 00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic
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Title The absorption spectrum of methyl orange Aim To prove beer lamberts law Introduction This experiment is to prove Beer lamberts law‚ which states that absorbance is proportional to concentration. Part one materials 1. Colorimeter 2. Methyl orange 3. Potassium permangate 4. Cuvette 5. Deionised water Part one methods 1. Rinse one cuvette with deionised water 2. Fill one cuvette with deionised water and “zero” the colorimeter machine 3. Fill the cuvette that
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22.12.2009 EN Official Journal of the European Union L 342/59 REGULATION (EC) No 1223/2009 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 30 November 2009 on cosmetic products (recast) (Text with EEA relevance) THE EUROPEAN PARLIAMENT AND THE COUNCIL OF THE EURO PEAN UNION‚ (5) Having regard to the Treaty establishing the European Commu nity‚ and in particular Article 95 thereof‚ Having regard to the proposal from the Commission‚ Having regard to the opinion of the European Economic
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which is strong acid-weak base titration and weak acid-strong base titration will be investigated. Materials Chemicals 0.1M Hydrochloric acid‚ 0.1M acetic acid‚ 0.1M sodium hydroxide‚ 0.1M ammonium hydroxide‚ phenolphthalein‚ screened methyl orange‚ methyl orange Apparatus 100mL Beakers‚ 250mL Conical flasks‚ 50mL Burettes‚ 25mL Pipettes‚ Pipette fillers‚ Funnels‚ pH meters Procedure The experiments are carried out according to the procedures that stated in Experiment (1): Investigation of the suitability
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An indicator is a dye which changes colour when put into an acid or a base. The different kinds of indicators are- Natural indicators- Litmus is a natural indicator‚ litmus solution is a purple dye which is extracted from a plant called lichen. Litmus turns red in acidic solutions and blue in basic solutions. Other examples are turmeric which contains a yellow dye‚ and red cabbage extract which is
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bromophenol blue. 2. To construct a standard concentration curve of bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange in a mixture. Materials and Methods: Refer to Biological Science practical manual page 5-11. Results: Part 1: Determination of Amax of bromophenol blue The wavelength with maximum absorbance reading form the graph is 590nm. Part 2: The effect
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