CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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to dye 3. 1 package RIT powder dye or 1/2 bottle RIT liquid dye 4. Rubber bands‚ string‚ or 2-inch wide strips of cloth for tying Instructions: 1. Gather the shirt into accordion-like folds‚ then fold the gathered fabric in half. Band tightly with cloth strips every 2 or 3 inches. 2. Before starting to dye‚ put on rubber gloves and prepare the dye bath. Dissolve 1 package RIT powder dye or 1/2 cup RIT liquid dye in about 2 gallons very hot water. For dark or bright colors‚ prepare dye bath
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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The earliest surviving examples of pre-Colombian tie-dye in Peru date from 500 to 800A.D. Their designs include small circles and lines‚ with bright colors including red‚ yellow‚ blue‚ and green.[4] Shibori includes a form of tie-dye that originated in Japan. It has been practiced there since at least the eighth century. Shibori includes a number of labor-intensive resist techniques including stitching elaborate patterns and tightly gathering the stitching before dyeing‚ forming intricate designs
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use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions: 1. Describe
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educate the public about the toxic dangers of floor wax no one seems to believe them due to the effectiveness of the modern floor wax but still its harming our environment. Our research aims to put these problems away through the use of eggplant leaves‚ by the use of eggplant leaves we can eliminate the problems of toxic materials exposing human and plants and making the floor wax safe to dispose at the environment because it can degrade over time plus making it 2 times more cheaper and the most important
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the cell. Some characteristics of cell membranes are discovered in this exercise. Background The following information is key for the interpretation of the results of this experiment 1. Neutral red is a vital stain. It stains cells without quickly killing them. Many biological stains kill living cells. 2. Neutral red goes through an obvious color change‚ from red at about pH 6.8 to yellow at pH 8.0. 3. A 1% solution of sodium bicarbonate has a pH of about 8.5. 4. Yeast cells have an internal
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Pamantasan ng Lungsod ng Marikina Brazil St. Greenheights Subd. Concepcion 1‚ Marikina City INVESTIGATORY PROJECT “Peel‚ da Banana Wax” MEMBERS: Alad‚ Mary Anne C. Aresgado‚ Jinky D. Casakit‚ Lowie L. Ducay‚ Gilrose T. Eroles‚ Ronalyn B. Gamboa‚ Geralyn S. Garcia‚ Christine O. Inocencio‚ Jellica C. Lobreo‚ Danica B. Lorayna‚ Ellen Grace L. Novelero‚ Annalyn R. Resuento‚ April Siena I. Mr. Ryan H. Lim ACKNOWLEDGEMENT The researchers would like to give their warm heart-felt
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Paulo César Stringheta‚ and Milton Cano Chauca Anthocyanin in spinach vine (Basella rubra) fruits Experimental t-test In conclusion‚ the pigment extracted from the fruits of spinach vine (B. rubra) was more stable at pH 5 and pH 6. Increases in temperature and the presence of light affected the stability of this pigment. Results demonstrate that this pigment exhibits good stability and could be applied to foods as a natural food color. The study is limited to the stability of anthocyanin in spinach
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